Anti-PGC1 alpha antibody
| Name | Anti-PGC1 alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab77210 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB FC ELISA |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 689-799 of Human PGC1 alpha with proprietary tag |
| Description | Mouse Monoclonal |
| Gene | PPARGC1A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PGC1 alpha antibody (ab77210) at 1 µg/ml + Recombinant fragment of human PGC1 alpha at 0.1 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
Anti-PGC1 alpha antibody (ab77210) at 1 µg/ml + PGC1 alpha transfected U2OS cell lysate at 25 µgSecondaryGoat anti-Mouse IgG (H&L)-HRP at 1/2500 dilutiondeveloped using the ECL technique
![Overlay histogram showing HEK293 cells stained with ab77210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77210, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10543_PGC1-alpha-Primary-antibodies-ab77210-3.jpg)
Overlay histogram showing HEK293 cells stained with ab77210 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77210, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.