All lanes : Anti-PHAPI2 / APRIL antibody [EPR14589] (ab184565) at 1/5000 dilutionLane 1 : LnCaP cell lysateLane 2 : PC3 cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue labeling PHAPI2 / APRIL with ab184565 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Flow cytometric analysis of PC3 cells (2% paraformaldehyde-fixed) labeling PHAPI2 / APRIL with ab184565 at 1/20 dilution (red) or Rabbit monoclonal IgG isotype control (green) followed by Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunofluorescent analysis of PC3 cells (4% pafarormaldehyde-fixed) labeling PHAPI2 / APRIL with ab184565 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and DAPI staining (blue).
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PHAPI2 / APRIL with ab184565 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.