Anti-PHD1/prolyl hydroxylase antibody
| Name | Anti-PHD1/prolyl hydroxylase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab80361 |
| Prices | $376.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Species Reactivities | Human, Orangutan |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PHD1/prolyl hydroxylase |
| Description | Rabbit Polyclonal |
| Gene | EGLN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PHD1/prolyl hydroxylase antibody (ab80361) at 1 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
ICC/IF image of ab80361 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab80361, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.