Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
| Name | Anti-PHD1/prolyl hydroxylase antibody [EPR2745] |
|---|---|
| Supplier | Abcam |
| Catalog | ab108980 |
| Prices | $388.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EPR2745 |
| Applications | WB IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues in Human PHD1/prolyl hydroxylase |
| Description | Rabbit Monoclonal |
| Gene | EGLN2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilutionLane 1 : A549 cell lysates Lane 2 : HeLa cell lysatesLane 3 : MCF-7 cell lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10851_PHD1prolyl-hydroxylase-Primary-antibodies-ab108980-1.jpg)
All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilutionLane 1 : A549 cell lysates Lane 2 : HeLa cell lysatesLane 3 : MCF-7 cell lysates Lysates/proteins at 10 µg per lane.SecondaryStandard HRP labelled goat anti-rabbit at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980
Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980
Immunofluorescent staining of HeLa cells using ab108980
Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
miR455 is linked to hypoxia signaling and is deregulated in preeclampsia. - miR455 is linked to hypoxia signaling and is deregulated in preeclampsia.
Lalevee S, Lapaire O, Buhler M. Cell Death Dis. 2014 Sep 4;5:e1408.
Differential expression of prolyl hydroxylase 1 in patients with ulcerative - Differential expression of prolyl hydroxylase 1 in patients with ulcerative
Van Welden S, Laukens D, Ferdinande L, De Vos M, Hindryckx P. J Inflamm (Lond). 2013 Nov 20;10(1):36.