Anti-PHD2 / prolyl hydroxylase antibody
| Name | Anti-PHD2 / prolyl hydroxylase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab4561 |
| Prices | $395.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF IP WB |
| Species Reactivities | Human |
| Antigen | A synthetic peptide made to an internal fragment (between aa residues 1-50) of human HIF prolyl hydroxylase 2 |
| Description | Rabbit Polyclonal |
| Gene | EGLN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-PHD2 / prolyl hydroxylase antibody (ab4561) at 1/1000 dilutionLane 1 : U2OS osteosarcoma cells (untreated) - whole cell lysateLane 2 : U2OS osteosarcoma cells (100uM DFO for 24 hours) - whole cell lysateLysates/proteins at 20 µg per lane.SecondaryHRP conjugated goat anti-rabbitdeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab4561 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4561, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Onconeuronal cerebellar degeneration-related antigen, Cdr2, is strongly expressed - Onconeuronal cerebellar degeneration-related antigen, Cdr2, is strongly expressed
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