Anti-PHD2 / prolyl hydroxylase antibody [366G/76/3]
| Name | Anti-PHD2 / prolyl hydroxylase antibody [366G/76/3] |
|---|---|
| Supplier | Abcam |
| Catalog | ab103432 |
| Prices | $384.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 366G/76/3 |
| Applications | IHC-P WB FC |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: MANDSGGPGGPSPSERDRQYCELCG , corresponding to amino acids 1-24 of Human PHD2/ prolyl hydroxylase Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | EGLN1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Overlay histogram showing SHSY-5Y cells stained with ab103432 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103432, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/10866_ab103432-1-ab103432FC.jpg)
Overlay histogram showing SHSY-5Y cells stained with ab103432 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab103432, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Use of novel monoclonal antibodies to determine the expression and distribution - Use of novel monoclonal antibodies to determine the expression and distribution
Soilleux EJ, Turley H, Tian YM, Pugh CW, Gatter KC, Harris AL. Histopathology. 2005 Dec;47(6):602-10.
Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the - Differential function of the prolyl hydroxylases PHD1, PHD2, and PHD3 in the
Appelhoff RJ, Tian YM, Raval RR, Turley H, Harris AL, Pugh CW, Ratcliffe PJ, Gleadle JM. J Biol Chem. 2004 Sep 10;279(37):38458-65. Epub 2004 Jul 7.
Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting - Genetic analysis of the role of the asparaginyl hydroxylase factor inhibiting
Stolze IP, Tian YM, Appelhoff RJ, Turley H, Wykoff CC, Gleadle JM, Ratcliffe PJ. J Biol Chem. 2004 Oct 8;279(41):42719-25. Epub 2004 Aug 9.