All lanes : Anti-PHD2 / prolyl hydroxylase antibody [mAbcam74796] (ab74796) at 5 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.