Anti-PHF8 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab36068
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB ICC/IF ICC/IF IHC-P ChIP
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human PHF8
• Description: Rabbit Polyclonal
• Gene: PHF8
• Conjugate: Unconjugated

Product images

All lanes : Anti-PHF8 antibody - ChIP Grade (ab36068) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

All lanes : Anti-PHF8 antibody - ChIP Grade (ab36068) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

ICC/IF image of ab36068 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36068, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HepG2 cells at 5µg/ml, and in 100% methanol fixed (5 min) Hek293 cells at 5µg/ml.

IHC image of ab36068 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab36068, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Product References

Genomic and proteomic dissection and characterization of the human sperm - Genomic and proteomic dissection and characterization of the human sperm

Castillo J, Amaral A, Azpiazu R, Vavouri T, Estanyol JM, Ballesca JL, Oliva R. Mol Hum Reprod. 2014 Nov;20(11):1041-53.

Oncogenic features of PHF8 histone demethylase in esophageal squamous cell - Oncogenic features of PHF8 histone demethylase in esophageal squamous cell

Sun X, Qiu JJ, Zhu S, Cao B, Sun L, Li S, Li P, Zhang S, Dong S. PLoS One. 2013 Oct 11;8(10):e77353.

The G2/M regulator histone demethylase PHF8 is targeted for degradation by the - The G2/M regulator histone demethylase PHF8 is targeted for degradation by the

Lim HJ, Dimova NV, Tan MK, Sigoillot FD, King RW, Shi Y. Mol Cell Biol. 2013 Nov;33(21):4166-80.

The histone demethylase PHF8 is essential for cytoskeleton dynamics. - The histone demethylase PHF8 is essential for cytoskeleton dynamics.

Asensio-Juan E, Gallego C, Martinez-Balbas MA. Nucleic Acids Res. 2012 Oct;40(19):9429-40.

Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial - Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial

Qi HH, Sarkissian M, Hu GQ, Wang Z, Bhattacharjee A, Gordon DB, Gonzales M, Lan F, Ongusaha PP, Huarte M, Yaghi NK, Lim H, Garcia BA, Brizuela L, Zhao K, Roberts TM, Shi Y. Nature. 2010 Jul 22;466(7305):503-7.