PHF8

Gene Symbol PHF8
Entrez Gene 23133
Alt Symbol JHDM1F, MRXSSD, ZNF422
Species Human
Gene Type protein-coding
Description PHD finger protein 8
Other Description histone lysine demethylase PHF8|jumonji C domain-containing histone demethylase 1F
Swissprots Q7Z6D4 Q9HAH2 Q5JPS3 Q5JPS0 Q5JPR9 Q5VUJ4 Q5JPS2 Q5H9U5 Q9UPP1 B3KMV4 B7Z911
Accessions EAW93167 EAW93168 EAW93169 EAW93170 Q9UPP1 AB029034 BAA83063 AF091081 AAC72950 AK021696 BAB13877 AK022788 BAG51116 AK027229 AK304272 BAH14147 BC017720 BC042108 BC053861 AAH53861 BE676640 CR933612 CAI45929 DA458639 DB079048 XM_005261996 XP_005262053 XM_005261997 XP_005262054 XM_005261999 XP_005262056 XM_005262000 XP_005262057 XM_006724585 XP_006724648 XM_011530778 XP_011529080 NM_001184896 NP_001171825 NM_001184897 NP_001171826 NM_001184898 NP_001171827 NM_015107 NP_055922
Function Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Acts as a coactivator of rDNA transcription, by activating polymerase I (pol I) mediated transcription of rRNA genes. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. May also have weak activity toward dimethylated H3 'Lys-36' (H3K36Me2), however, the relevance of this result remains unsure in
Subcellular Location Nucleus. Nucleus, nucleolus. Note=Recruited to H3K4me3 sites on chromatin during interphase. Dissociates from chromatin when cells enter mitosis.

PHF8 (P-15) - sc-131751 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PHF8 antibody - ab84779 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab84779, at a 1/100 dilution, staining PHF8 in formalin fixed, paraffin embedded human breast carcinoma by Immunohistochemistry using DAB staining.

Anti-PHF8 antibody - ab176371 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PHF8 antibody (ab176371) at 1/1000 dilutionLane 1 : HeLa total cell extractLane 2 : Jurkat total cell extract

Anti-PHF8 antibody - ChIP Grade - ab36068 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PHF8 antibody - ChIP Grade (ab36068) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PHF8 Antibody (aa925-975) - LS-C126129 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF8 Antibody (aa650-700) - LS-C126130 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF8 Antibody - LS-C347723 from LifeSpan Bioscience

Prices $345.00
Sizes 25 µg
Host Rabbit
Clonality Polyclonal

Anti-PHF8 Antibody (aa925-975) - LS-C288554 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PHF8 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, HeLa (RIPA), 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-PHF8 antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-PHF8 Antibody (aa650-700) - LS-C286580 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Mouse PHF8 by Immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-PHF8 used at a dilution of 1:100.

Anti-PHF8 Antibody (aa650-700) IHC-plus™ - LS-B11788 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

PHF8 Antibody - NB100-93314 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: PHF8 Antibody [NB100-93314] - Section of human prostate carcinoma (left) and mouse teratoma (right). Dilution: 1:200 (1 ug/ml).

PHF8 Antibody - NB100-93313 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry: PHF8 Antibody [NB100-93313] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- PHF8 used at a dilution of 1:200 (1 ug/ml). Detection: DAB

PHF8 Antibody - NBP1-83079 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PHF8 Polyclonal Antibody - bs-8176R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PHF8 (N-term) antibody produced in rabbit - SAB1300509 from Sigma-Aldrich

Prices $291.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PHF8 antibody produced in rabbit - HPA038779 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PHF8: <B>Cat. No. HPA038779</B>: Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neuronal cells.

PHF8 / ZNF422 antibody - PAB14120 from Acris Antibodies

Prices $420.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
E14TG2A ( mouse embryonic stem cells ) were transfected with a conditional allele of PHF8. Nuclear extracts ( 135 µg ) from wild type cells ( WT ) and from 2 clones ( F1, B8 ) with an active and a targeted allele ( F1ko, B8ko ), respectively, were analysed by Western blot using PHF8 polyclonal antibody ( Cat # PAB14120 ). The antibody was diluted 1 : 1,000.

Anti-PHF8 Antibody - AC11-0194 from Abcore

Prices $325.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

PHF8 Antibody - E220012-1 from Enogene

Prices $145.00
Sizes 50μg/50μl

PHF8 Antibody - E220012-2 from Enogene

Prices $225.00
Sizes 100μg/100μl

PHF8 antibody (pAb) - 39711 from ActiveMotif

Prices $95.00, $410.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Polyclonal
PHF8 pAb tested by Western blot. Nuclear extract of HeLa cells (20 µg) was probed with PHF8 pAb (1: 500 dilution).

Anti-PHF8 (internal region) polyclonal antibody - DPABH-11942 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

PHF8 Antibody - A301-772A from Bethyl Labs

Prices $99.00, $229.00, $339.00
Sizes 20 µl (2 blots), 100 µl (10 blots), 100 µl (0.2 mg/ml)
Host Rabbit
Clonality Polyclonal

PHF8 Antibody - A301-773A from Bethyl Labs

Prices $99.00, $229.00, $339.00
Sizes 20 µl (2 blots), 100 µl (10 blots), 100 µl (0.2 mg/ml)
Host Rabbit
Clonality Polyclonal

PHF8 IHC Antibody - IHC-00343 from Bethyl Labs

Prices $99.00, $360.00
Sizes 10 µl (5+ slides), 100 µl (50+ slides)
Host Rabbit
Clonality Polyclonal
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