Anti-Phospholipase A2 (phospho S505) antibody
| Name | Anti-Phospholipase A2 (phospho S505) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53105 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F WB IHC-P ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic phosphopeptide derived from human Phospholipase A2 around the phosphorylation site of serine 505 (P-L-SP-P-L) |
| Description | Rabbit Polyclonal |
| Gene | PLB1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Phospholipase A2 (phospho S505) antibody (ab53105) at 1/300 dilutionLane 1 : extracts from HeLa cells treated with TNF-a (20ng/ml, 30mins)Lane 2 : extracts from HeLa cells treated with TNF-a (20ng/ml, 30mins) with immunising peptide
ab53105 at 1/50 dilution staining Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence and presence of the immunising peptide.
ICC/IF image of ab53105 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53105, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
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Singh NK, Hansen DE 3rd, Kundumani-Sridharan V, Rao GN. Blood. 2013 Mar 7;121(10):1911-23.
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Estevez B, Stojanovic-Terpo A, Delaney MK, O'Brien KA, Berndt MC, Ruan C, Du X. Blood. 2013 May 30;121(22):4586-94.
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Ma L, Nagai J, Chun J, Ueda H. Mol Pain. 2013 Jun 17;9:29.
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Manosalva I, Gonzalez A. Biol Reprod. 2009 Dec;81(6):1164-71.