Anti-Phospholipase A2 antibody (ab135825) at 1/100 dilution + NCI-H460 cell lysate at 35 µg
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labelling Phospholipase A2 with ab135825 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
Immunofluorescent analysis of NCI-H460 cells labelling Phospholipase A2 with ab135825 1/10 dilution followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of NCI-H292 cells labelling Phospholipase A2 with ab135825 dilution 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.