Anti-PI 3 Kinase Class 3 antibody
| Name | Anti-PI 3 Kinase Class 3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5451 |
| Prices | $385.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to amino acids 39-54 of Human PI3KC 3 |
| Description | Rabbit Polyclonal |
| Gene | PIK3C3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab5451 staining PI 3 Kinase Class 3 in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
ICC/IF image of ab5451 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5451, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.