ICC/IF image of ab62901 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab62901 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab62901 at 1/50 - 1/100 dilution, staining PI 3 Kinase p85 beta in human brain by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue. ab62901 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
All lanes : Anti-PI 3 Kinase p85 beta antibody (ab62901) at 1/100 dilutionLane 1 : 293 cell lysates, nontransfectedLane 2 : 293 cell lysates, transiently transfected with the PI 3 Kinase p85 beta gene Lysates/proteins at 2 µg per lane.