All lanes : Anti-PIAS2 antibody (ab155556) at 1/1000 dilutionLane 1 : Jurkat whole cell lysateLane 2 : Raji whole cell lysateLane 3 : K562 whole cell lysateLane 4 : THP-1 whole cell lysateLane 5 : HL-60 whole cell lysateLane 6 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.
All lanes : Anti-PIAS2 antibody (ab155556) at 1/10000 dilutionLane 1 : NIH-3T3 whole cell lysateLane 2 : JC whole cell lysateLane 3 : BCL-1 whole cell lysateLysates/proteins at 30 µg per lane.
Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue labeling PIAS2 with ab155556 at 1/500 dilution.