PIAS2

Gene Symbol PIAS2
Entrez Gene 9063
Alt Symbol ARIP3, DIP, MIZ, MIZ1, PIASX, PIASX-ALPHA, PIASX-BETA, SIZ2, ZMIZ4
Species Human
Gene Type protein-coding
Description protein inhibitor of activated STAT, 2
Other Description DAB2-interacting protein|E3 SUMO-protein ligase PIAS2|androgen receptor-interacting protein 3|msx-interacting zinc finger protein|protein inhibitor of activated STAT X|zinc finger, MIZ-type containing 4
Swissprots O75928 O75927 Q96KE3 Q96BT5
Accessions EAX01484 EAX01485 EAX01486 EAX01487 EAX01488 O75928 AA701927 AF077953 AAC36704 AF077954 AAC36705 AF361054 AAK48938 AK294801 BAG57921 AK308143 AW016227 BC015190 AAH15190 BC111060 AAI11061 BG718291 BX646432 DQ896421 ABM87420 EU176432 ABW03883 XM_005258377 XP_005258434 XM_005258379 XP_005258436 XM_005258381 XP_005258438 XM_005258382 XP_005258439 XM_006722571 XP_006722634 XM_006722572 XP_006722635 XM_006722573 XP_006722636 XM_011526258 XP_011524560 XM_011526259 XP_011524561 NM_004671 NP_004662 NM_173206 NP_775298
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2- alpha. Isoform PIAS2-alpha sumoylates PML at'Lys-65' and 'L
Subcellular Location Nucleus speckle. Nucleus, PML body. Note=Colocalizes at least partially with promyelocytic leukemia nuclear bodies (PML NBs).
Tissue Specificity Mainly expressed in testis. Isoform 3 is expressed predominantly in adult testis, weakly in pancreas, embryonic testis and sperm, and at very low levels in other organs. {ECO:0000269|PubMed:11439351, ECO:0000269|PubMed:15301740, ECO:0000269|PubMed:9920921}.
Top Pathways Ubiquitin mediated proteolysis, Jak-STAT signaling pathway, Small cell lung cancer

PIASx (C-20) - sc-18245 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIASx (C-20): sc-18245. Western blot analysis of PIASx expression in non-transfected: sc-110760 (A) and mouse PIASx transfected: sc-111287 (B) 293 whole cell lysates.

PIASx (D-12) - sc-166494 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PIASx (D-12): sc-166494. Western blot analysis of PIASx expression in non-transfected: sc-110760 (A) and mouse PIASx transfected: sc-111287 (B) 293 whole cell lysates.

PIASx (L-19) - sc-30878 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIASx (L-19): sc-30878. Western blot analysis of PIAS 1 expression in non-transfected: sc-117752 (A) and mouse PIAS 1 transfected: sc-122561 (B) 293T whole cell lysates.

PIASx (S-15) - sc-30879 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIASx (S-15): sc-30879. Western blot analysis of PIASx expression in non-transfected: sc-110760 (A) and mouse PIASx transfected: sc-111287 (B) 293 whole cell lysates.

Anti-PIAS2 antibody - ab105361 from Abcam

Host Rabbit
Clonality Polyclonal
Immunofluorescence of PIAS2 in rat brain tissue with ab105361 at 20 ug/mL.

Anti-PIAS2 antibody - ab155556 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PIAS2 antibody (ab155556) at 1/1000 dilutionLane 1 : Jurkat whole cell lysateLane 2 : Raji whole cell lysateLane 3 : K562 whole cell lysateLane 4 : THP-1 whole cell lysateLane 5 : HL-60 whole cell lysateLane 6 : NCI-H929 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-PIAS2 antibody - ab88598 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PIAS2 antibody (ab88598) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

Anti-PIAS2 antibody - ab4902 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of anti-PIASx1/2 Pab (ab4902) in Y79 cell lysate. PIASx1/2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Anti-PIAS2 antibody - ab58404 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PIAS2 antibody (ab58404) at 1/500 dilutionLane 1 : Cos7 cell extractLane 2 : Cos7 cell extract with peptide

Anti-PIAS2 antibody [EPR2582(2)] - ab126601 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2582(2)
All lanes : Anti-PIAS2 antibody [EPR2582(2)] (ab126601) at 1/1000 dilutionLane 1 : HepG2 cell lysateLane 2 : Human fetal kidney tissue lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-Rabbit HRP at 1/2000 dilution

Anti-PIAS2 / PIASX Antibody (aa431-445) IHC-plus™ - LS-B3240 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-PIAS2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3240 concentration 75 ug/ml.

Anti-PIAS2 / PIASX Antibody - LS-C334194 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence analysis of U20S cell using PIAS2 antibody. Blue: DAPI for nuclear staining.

Anti-PIAS2 / PIASX Antibody (C-Terminus) - LS-C31336 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 Cell Lysate

Anti-PIAS2 / PIASX Antibody (C-Terminus) - LS-C31338 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
HepG2 Cell Lysate

Anti-PIAS2 / PIASX Antibody (C-Terminus) - LS-C30501 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Mouse Pancreas

Anti-PIAS2 / PIASX Antibody (aa58-107) - LS-C81820 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Fetal liver lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.

Anti-PIAS2 / PIASX Antibody (aa1-550) - LS-C126135 from LifeSpan Bioscience

Prices $605.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-PIAS2 / PIASX Antibody (aa518-547) - LS-C98797 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-PIAS2 / PIASX Antibody (C-Terminus, AP) - LS-C245314 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PIAS2 / PIASX Antibody (C-Terminus, APC) - LS-C245315 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PIAS2 / PIASX Antibody (C-Terminus, Biotin) - LS-C245316 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PIAS2 / PIASX Antibody (aa81-130) - LS-C116154 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIAS2 antibody LS-C116154 Western blot of Mouse Thymus lysate.

Anti-PIAS2 / PIASX Antibody (aa51-100) - LS-C146213 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIAS2 antibody LS-C146213 Western blot of Mouse Heart lysate. Antibody concentration 1 ug/ml.

Anti-PIAS2 / PIASX Antibody (C-Terminus, FITC) - LS-C245317 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PIAS2 / PIASX Antibody (C-Terminus, PE) - LS-C245318 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
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