Anti-PIAS3 antibody
| Name | Anti-PIAS3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab22856 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-P |
| Species Reactivities | Mouse, Human, Rat, Bovine, Dog |
| Antigen | Synthetic peptide: HYGPSVITSL DEQDALGHFF QYRGTPSHFL GPLAPTLGSS HCSATPAPPP, corresponding to a sequence within C terminal amino acids 541-590 of Human PIAS3 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | PIAS3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-PIAS3 antibody (ab22856) at 0.004 µg/ml + HepG2 cell lysateSecondaryHRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 Performed under reducing conditions.Observed band size : 68 kDa (why is the actual band size different from the predicted?)
ab22856, at a 1/125-250 dilution, staining human PIAS3 in human liver by Immunohistochemistry, Paraffin embedded tissue. Positive stained hepatocytes indicated by arrows. Magnification 400 x.
Immunocytochemistry/ Immunofluorescence analysis of MCF-7 cells transfected with either control siRNA or siRNAs targeting PIAS3. Complexes of ErbB4 and PIAS3 were visualized with antibodies against ErbB4 and PIAS3 (ab22856) using in situ PLA. Each red dot represents a single interaction. The nuclei were stained with DAPI (blue).
Product References
Protein inhibitor of activated STAT3 (PIAS3) protein promotes SUMOylation and - Protein inhibitor of activated STAT3 (PIAS3) protein promotes SUMOylation and
Sundvall M, Korhonen A, Vaparanta K, Anckar J, Halkilahti K, Salah Z, Aqeilan RI, Palvimo JJ, Sistonen L, Elenius K. J Biol Chem. 2012 Jun 29;287(27):23216-26.
The intracellular delivery of a recombinant peptide derived from the acidic - The intracellular delivery of a recombinant peptide derived from the acidic
Borghouts C, Tittmann H, Delis N, Kirchenbauer M, Brill B, Groner B. Mol Cancer Res. 2010 Apr;8(4):539-53.
Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins. - Regulation of MBD1-mediated transcriptional repression by SUMO and PIAS proteins.
Lyst MJ, Nan X, Stancheva I. EMBO J. 2006 Nov 15;25(22):5317-28. Epub 2006 Oct 26.