PIAS3

Gene Symbol PIAS3
Entrez Gene 10401
Alt Symbol ZMIZ5
Species Human
Gene Type protein-coding
Description protein inhibitor of activated STAT, 3
Other Description E3 SUMO-protein ligase PIAS3|protein inhibitor of activated STAT protein 3|zinc finger, MIZ-type containing 5
Swissprots Q9UFI3 Q9Y6X2
Accessions EAW71429 EAW71430 EAW71431 Q9Y6X2 AB021868 BAA78533 AK027557 BAG51345 AK125158 AK294231 BAG57533 AK304039 BAG64950 AL122061 CAB59241 AL133016 CAB61357 BC001154 AAH01154 BC030556 AAH30556 BE247489 BT007036 AAP35684 CR457090 CAG33371 DQ893542 ABM84468 DQ893796 ABM84722 DQ895037 ABM85963 EU176644 ABW03445 NM_006099 NP_006090
Function Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. {ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:9388184}.
Subcellular Location Cytoplasm. Nucleus. Note=Mainly cytoplasmic but shuttles between the nucleus and cytoplasm. Transported to the nucleus on interaction with phosphorylated STAT3. Colocalizes with GFI1 in nuclear dots (By similarity). {ECO:0000250}.
Tissue Specificity Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. {ECO:0000269|PubMed:9388184}.
Top Pathways Ubiquitin mediated proteolysis, Jak-STAT signaling pathway

PIAS3 (D5F9) XP ® Rabbit mAb - 9042 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D5F9
Western blot analysis of extracts from RD cells, transfected with 100 nM SignalSilence ® Control siRNA (Unconjugated) #6568 (-), SignalSilence ® PIAS3 siRNA I #9073 (+), or SignalSilence ® PIAS3 siRNA II #9031 (+), using PIAS3 (D5F9) XP ® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). The PIAS3 (D5F9) XP ® Rabbit mAb confirms silencing of PIAS3 expression, while the β-Actin (D6A8) Rabbit mAb is used as a loading control.

PIAS3 Antibody - 4164 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analyis of extracts from various cell lines, including COS-7 cells, mock transfected or transfected with mouse PIAS1 or PIAS3, using PIAS3 Antibody. Overexpressed PIAS3 runs as a doublet as previously reported (15).

PIAS 3 (C-12) - sc-46682 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PIAS 3 (C-12): sc-46682. Western blot analysis of human recombinant PIAS 3 representing amino acids 453-550 (A) and 451-619 (B).

PIAS 3 (E-3) - sc-48339 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PIAS 3 (E-3): sc-48339. Western blot analysis of human recombinant PIAS 3 representing amino acids 453-550 (A) and 451-619 (B). Note non cross-reactivity of protein fragment in lane A.

PIAS 3 (H-169) - sc-14017 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PIAS 3 (H-169): sc-14017. Western blot analysis of PIAS 3 expression in HeLa (A), K-562 (B) and HL-60 (C) whole cell lysates.

PIAS 3 (M-20) - sc-8154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PIAS3 + PIAS1 + PIAS2 antibody [EPR2581Y] - ab77231 from Abcam

Prices $99.00, $357.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2581Y
All lanes : Anti-PIAS3 + PIAS1 + PIAS2 antibody [EPR2581Y] (ab77231) at 1/50000 dilutionLane 1 : Daudi cell lysateLane 2 : 293 cell lysateLane 3 : Jurkat cell lysateLane 4 : SW40 cell lysateLane 5 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-PIAS3 antibody - ab22856 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PIAS3 antibody (ab22856) at 0.004 µg/ml + HepG2 cell lysateSecondaryHRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 Performed under reducing conditions.Observed band size : 68 kDa (why is the actual band size different from the predicted?)

Anti-PIAS3 antibody - ab58406 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue using ab58406 at 1/50-1/100 dilution. Samples were treated -/+ peptide.

Anti-PIAS3 antibody - ab105178 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PIAS3 antibody (ab105178) at 1 µg/mlLane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PIAS3 antibody - ab119106 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab119106, at 5 µg/ml, staining PIAS3 in Formalin-fixed, Paraffin-embedded Human Prostate tissue by Immunohistochemistry.

Anti-PIAS3 antibody - ab195686 from Abcam

Prices $370.00
Sizes 250 µg
Host Rabbit
Clonality Polyclonal
Anti-PIAS3 antibody (ab195686) at 1/1000 dilution + Human fetal intestine lysate

Anti-PIAS3 antibody - ab56698 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56698 PIAS3 antibody at 1ug/ml.

Anti-PIAS3 antibody [PIA3] - ab59547 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone PIA3

Anti-PIAS3 antibody - C-terminal - ab135131 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-PIAS3 antibody - C-terminal (ab135131) at 1/1000 dilution + HepG2 total cell extract

Anti-PIAS3 Antibody (C-Terminus) IHC-plus™ - LS-B5091 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PIAS3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5091 concentration 5 ug/ml.

Anti-PIAS3 Antibody (aa274-521) - LS-C178737 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PIAS3 Antibody - LS-C346210 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence blot of U20S cell using PIAS3 antibody.

Anti-PIAS3 Antibody (Phe25) - LS-C176343 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
IHC of PIAS3 (F25) pAb in paraffin-embedded human lung carcinoma tissue.

Anti-PIAS3 Antibody (aa10-59) IHC-plus™ - LS-B7646 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PIAS3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7646 concentration 10 ug/ml.

Anti-PIAS3 Antibody (aa217-500) - LS-C311733 from LifeSpan Bioscience

Prices $250.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PIAS3 Antibody (aa395-444) - LS-C109712 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIAS3 antibody LS-C109712 Western blot of Fetal Spleen lysate.

Anti-PIAS3 Antibody (aa548-597) - LS-C31333 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
PIAS3 antibody LS-C31333 was used in IHC to stain formalin-fixed, paraffin-embedded human liver.

PIAS3 Antibody - NBP1-77160 from Novus Biologicals

Prices $329.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western Blot: PIAS3 Antibody [NBP1-77160] - K562 cell lysate with PIAS3 antibody at 1 ug/mL.

PIAS3 Antibody - AF5120 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal
Western blot shows nuclear extracts of HepG2 human hepatocellular carcinoma cell line, HEK293 human embryonic kidney cell line, and MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PIAS3 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5120) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PIAS3 at approximately 65-75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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