ab113796 staining PICALM in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fresh-frozen, sliced to 18 microns, fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 2.5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 0.15% Triton + 1.5% normal Horse serum) for 20 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/400) was used as the secondary antibody.See Abreview
ab113796, at 5 µg/ml, staining PICALM in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry