PICALM

Gene Symbol PICALM
Entrez Gene 8301
Alt Symbol CALM, CLTH, LAP
Species Human
Gene Type protein-coding
Description phosphatidylinositol binding clathrin assembly protein
Other Description clathrin assembly lymphoid myeloid leukemia protein|phosphatidylinositol-binding clathrin assembly protein
Swissprots E9PN05 Q13492 B4DTM3 Q4LE54 O60700 Q6GMQ6 F8VPG7 Q86XZ9
Accessions AAN03747 CCQ43392 EAW75118 EAW75119 EAW75120 EAW75121 EAW75122 EAW75123 EAW75124 EAW75125 EAW75126 EAW75127 EAW75128 Q13492 AB210017 BAE06099 AB451308 BAG70122 AF060939 AAC16711 AF060940 AAC16712 AK128786 AK291414 BAF84103 AK297062 BAG59583 AK300275 BAG62035 AY326466 AAR99516 BC048259 AAH48259 BC064357 AAH64357 BC073961 AAH73961 CA415536 CN310579 DC300074 EF051633 ABK54356 U45976 AAB07762 XM_005274322 XP_005274379 XM_005274323 XP_005274380 XM_005274324 XP_005274381 XM_005274325 XP_005274382 XM_005274326 XP_005274383 XM_005274327 XP_005274384 XM_005274328 XP_005274385 XM_005274329 XP_005274386 XM_005274330 XP_005274387 XM_005274331 XP_005274388 XM_005274332 XP_005274389 XM_005274333 XP_005274390 XM_005274334 XP_005274391 XM_005274335 XP_005274392 XM_005274336 XP_005274393 XM_005274337 XP_005274394 XM_005274340 XP_005274397 XM_006718699 XP_006718762 XM_006718700 XP_006718763 XM_006718701 XP_006718764 XM_011545293 XP_011543595 NM_001008660 NP_001008660 NM_001206946 NP_001193875 NM_001206947 NP_001193876 NM_007166 NP_009097
Function Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. {ECO:0000269|PubMed:10436022}.
Subcellular Location Membrane, clathrin-coated pit. Golgi apparatus. Cytoplasmic vesicle, clathrin-coated vesicle. Nucleus. Note=Colocalized with clathrin in the Golgi area. Interaction with FAM64A may target PICALM to the nucleus in some cells.
Tissue Specificity Expressed in all tissues examined. {ECO:0000269|PubMed:8643484}.

CALM (F-3) - sc-166883 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CALM (F-3): sc-166883. Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.

CALM (G-8) - sc-390580 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CALM (G-8): sc-390580. Western blot analysis of CALM expression in HeLa whole cell lysate.

CALM (D-8) - sc-166522 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CALM (D-8): sc-166522. Western blot analysis of CALM expression in non-transfected 293T: sc-117752 (A), human CALM transfected 293T: sc-111583 (B) and K-562 (C) whole cell lysates.

CALM (G-17) - sc-5395 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CALM (G-17): sc-5395. Western blot analysis of CALM expression in HeLa (A), A-431 (B), K-562 (C) and KNRK (D) whole cell lysates.

CALM (A-2) - sc-271224 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
CALM (A-2): sc-271224. Western blot analysis of CALM expression in 293T whole cell lysate.

CALM (H-134) - sc-292597 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
CALM (H-134): sc-292597. Western blot analysis of CALM expression in non-transfected 293T: sc-117752 (A), human CALM transfected 293T: sc-111583 (B), KNRK (C) and NIH/3T3 (D) whole cell lysates.

CALM (C-18) - sc-6433 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CALM (C-18): sc-6433. Western blot analysis of CALM expression in A-431 (A), HeLa (B), Jurkat (C) and HL-60 (D) whole cell lysates.

CALM (S-19) - sc-5396 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
CALM (S-19): sc-5396. Western blot analysis of CALM expression in K-562 (A) and KNRK (B) whole cell lysates.

Anti-PICALM antibody - ab113796 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab113796 staining PICALM in Rat brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fresh-frozen, sliced to 18 microns, fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 2.5% serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in PBS + 0.15% Triton + 1.5% normal Horse serum) for 20 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit polyclonal (1/400) was used as the secondary antibody.See Abreview

Anti-PICALM antibody - ab106409 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.

Anti-PICALM antibody [EPR12177] - ab172962 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR12177
All lanes : Anti-PICALM antibody [EPR12177] (ab172962) at 1/1000 dilutionLane 1 : HepG2 lysateLane 2 : HeLa lysateLane 3 : A431 lysateLane 4 : Human fetal brain lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-PICALM / CALM Antibody (aa438-487) - LS-C146567 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PICALM antibody LS-C146567 Western blot of Jurkat Cell lysate. Antibody concentration 1 ug/ml.

Anti-PICALM / CALM Antibody (aa277-326) - LS-C146566 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PICALM antibody LS-C146566 Western blot of Fetal Heart lysate. Antibody concentration 1 ug/ml.

Anti-PICALM / CALM Antibody IHC-plus™ - LS-B10874 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-PICALM / CALM antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10874 concentration 7.5 ug/ml.

Anti-PICALM / CALM Antibody - LS-C155608 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Sample (30 ug of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE PICALM antibody diluted at 1:1000

Anti-PICALM / CALM Antibody (N-Terminus) IHC-plus™ - LS-B5065 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PICALM antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5065 concentration 5 ug/ml.

PICALM Antibody - NBP1-86659 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PICALM Antibody [NBP1-86659] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

PICALM Antibody - NBP1-86658 from Novus Biologicals

Prices $129.00, $419.00
Sizes 25 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PICALM Antibody [NBP1-86658] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).

PICALM Antibody - NBP2-19825 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PICALM Antibody [NBP2-19825] - Sample (30 ug of whole cell lysate) A: A549 7. 5% SDS PAGE gel, diluted at 1:2000.

PICALM Antibody - NBP2-19824 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PICALM Antibody [NBP2-19824] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:1000.

PICALM Antibody - NBP1-72144 from Novus Biologicals

Prices $399.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: PICALM Antibody [NBP1-72144] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

PICALM Antibody - H00008301-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

PICALM/CALM Polyclonal Antibody - bs-11665R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PICALM/CALM Polyclonal Antibody, Unconjugated (bs-11665R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

PICALM/CALM Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated - bs-11665R-A350 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PICALM/CALM Polyclonal Antibody, ALEXA FLUOR® 488 Conjugated - bs-11665R-A488 from Bioss

Prices $369.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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