Immunofluorescence of PICALM in Human brain tissue with ab106409 at 20 ug/mL.
ICC/IF image of PICALM staining on blood smear using ab106409 (1:200). The blood film was fixed in paraformaldehyde and permeabilized using 0.1% TritonX in 0.1% PBS. The cells were then blocked using 10% donkey serum for 1 hour at 24°C. ab106409 was diluted 1200 using and incubated with the cells for 4 hours at 24°C. The secondary antibody used was donkey polyclonal to rabbit IgG conjugated to Alexa Fluor 488 (1:1000)
All lanes : Anti-PICALM antibody (ab106409) at 1 µg/mlLane 1 : EL4 cell lysateLane 2 : EL4 cell lysate with immunizing peptideLysates/proteins at 15 µg per lane.