Anti-PICK1 antibody
| Name | Anti-PICK1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3420 |
| Prices | $401.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF IP WB ICC/IF ICC/IF IHC-P |
| Species Reactivities | Mouse, Rat, Human, Primate, Bovine, Monkey |
| Antigen | Synthetic peptide corresponding to Mouse PICK1 aa 1-18 |
| Description | Rabbit Polyclonal |
| Gene | PICK1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab3420 staining PICK1 in mouse Hippocampal neurons by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.1% saponin/PBS prior to blocking in 3% BSA for 1 hour at room temperature. The primary antibody was diluted 1/300 in 3% BSA, 0.1% saponin /PBS and incubated with the sample for 18 hours at 4 °C. The secondary antibody was Alexa Fluor® 455-conjugated Donkey anti-Rabbit polyclonal, diluted 1/1000.
Lane 1 : An myc antibody in PBST for 16 hours at 4°CLane 2 : Anti-PICK1 antibody (ab3420) at 1/500 dilution (in PBST for 16 hours at 4°C)Lane 1 : Transfected CHO cells expressing human Pick1 tagged with mycLane 2 : Transfected CHO cells expressing human Pick1 tagged with mycLysates/proteins at 20 µg per lane.SecondaryLane 2 : An HRP-conjugated goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : ~52 kDa (why is the actual band size different from the predicted?)Additional bands at : ~40 kDa. We are unsure as to the identity of these extra bands.Exposure time : 2 minutesThis image is courtesy of an anonymous AbreviewBlocking Step: 5% Milk for 1 hour at 20°CSee Abreview
IHC image of ab3420 staining in rat adult brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3420, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab3420 staining PICK1 in CHO cells stably transfected with Human PICK1 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in 0.5% Triton X-100 prior to blocking in 10% serum for 1 hour at room temperature. The primary antibody was diluted 1/1000 in PBS, 0.5% Triton, 10% FCS and incubated with the sample for 2 hours at °C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.See Abreview
Western blot of PICK1 on AtT20 cell lysate using ab3420.
Product References
Pathogenic disruption of DISC1-serine racemase binding elicits schizophrenia-like - Pathogenic disruption of DISC1-serine racemase binding elicits schizophrenia-like
Ma TM, Abazyan S, Abazyan B, Nomura J, Yang C, Seshadri S, Sawa A, Snyder SH, Pletnikov MV. Mol Psychiatry. 2013 May;18(5):557-67.
Identification of a small-molecule inhibitor of the PICK1 PDZ domain that - Identification of a small-molecule inhibitor of the PICK1 PDZ domain that
Thorsen TS, Madsen KL, Rebola N, Rathje M, Anggono V, Bach A, Moreira IS, Stuhr-Hansen N, Dyhring T, Peters D, Beuming T, Huganir R, Weinstein H, Mulle C, Stromgaard K, Ronn LC, Gether U. Proc Natl Acad Sci U S A. 2010 Jan 5;107(1):413-8.