All lanes : Anti-PIP5K2 alpha antibody [3A9] (ab117967) at 1/500 dilutionLane 1 : HEK293T cells transfected with pCMV6-ENTRY controlLane 2 : HEK293T cells transfected with pCMV6-ENTRY PIP5K2 alpha cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-PIP5K2 alpha antibody [3A9] (ab117967) at 1/200 dilutionLane 1 : Extracts from HepG2 cell lineLane 2 : Extracts from HeLa cell lineLane 3 : Extracts from SYT2 cell lineLane 4 : Extracts from A549 cell lineLane 5 : Extracts from COS7 cell lineLane 6 : Extracts from Jurkat cell lineLane 7 : Extracts from MDCK cell lineLane 8 : Extracts from PC12 cell lineLane 9 : Extracts from MCF7 cell lineLysates/proteins at 35 µg per lane.
Flow cytometric analysis of HEK293T cells transfected with either PIP5K2 alpha overexpress plasmid (Red) or empty vector control plasmid (Blue), immunostained with ab117967 at a 1/100 dilution.
Flow cytometric analysis of Hela cells using ab117967 at a 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometric analysis of Jurkat cells using ab117967 at a 1/100 dilution (Red), compared to a nonspecific negative control antibody (Blue).