All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : LnCaP cell lysateLane 3 : HeLa cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
All lanes : Anti-Pirh2 antibody [EPR14980] (ab189247) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : Raw 264.7 cell lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Pirh2 with ab189247 at 1/50 dilution. The slide is counterstained with Hematoxylin.
Immunofluorescence analysis of 4% paraformaldehyde-fixed HeLa cells labeling Pirh2 with ab189247 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with DAPI (blue).