Anti-PIWIL1 antibody
| Name | Anti-PIWIL1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12337 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from within residues 800 - 900 of Human PIWIL1 |
| Description | Rabbit Polyclonal |
| Gene | PIWIL1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Image courtesy of Human Protein Atlas ab12337 staining PIWI Human female colon, showing a distinct and strong staining pattern at glandular cells. Paraffin embedded human colon tissue was incubated with ab12337 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab12337 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
All lanes : Anti-PIWIL1 antibody (ab12337) at 1 µg/mlLane 1 : Testis (Mouse) Tissue LysateLane 2 : Liver (Mouse) Tissue LysateLane 3 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)Lane 4 : Liver (Rat) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
ICC/IF image of ab12337 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12337 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![PIWIL1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to PIWIL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12337.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 99kDa: PIWIL1](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12298_PIWIL1-Primary-antibodies-ab12337-4.jpg)
PIWIL1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to PIWIL1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab12337.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 99kDa: PIWIL1
Product References
AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during - AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during
Xu K, Yang L, Zhao D, Wu Y, Qi H. Biol Reprod. 2014 Jun;90(6):119.
Reduced pachytene piRNAs and translation underlie spermiogenic arrest in - Reduced pachytene piRNAs and translation underlie spermiogenic arrest in
Castaneda J, Genzor P, van der Heijden GW, Sarkeshik A, Yates JR 3rd, Ingolia NT, Bortvin A. EMBO J. 2014 Sep 17;33(18):1999-2019.
Evidence that RASSF1C stimulation of lung cancer cell proliferation depends on - Evidence that RASSF1C stimulation of lung cancer cell proliferation depends on
Reeves ME, Firek M, Chen ST, Amaar YG. PLoS One. 2014 Jul 9;9(7):e101679.
Hiwi mediated tumorigenesis is associated with DNA hypermethylation. - Hiwi mediated tumorigenesis is associated with DNA hypermethylation.
Siddiqi S, Terry M, Matushansky I. PLoS One. 2012;7(3):e33711.
A novel requirement in mammalian spermatid differentiation for the DAZ-family - A novel requirement in mammalian spermatid differentiation for the DAZ-family
VanGompel MJ, Xu EY. Hum Mol Genet. 2010 Jun 15;19(12):2360-9.
Mouse MOV10L1 associates with Piwi proteins and is an essential component of the - Mouse MOV10L1 associates with Piwi proteins and is an essential component of the
Zheng K, Xiol J, Reuter M, Eckardt S, Leu NA, McLaughlin KJ, Stark A, Sachidanandam R, Pillai RS, Wang PJ. Proc Natl Acad Sci U S A. 2010 Jun 29;107(26):11841-6. doi:
Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to - Mouse Piwi interactome identifies binding mechanism of Tdrkh Tudor domain to
Chen C, Jin J, James DA, Adams-Cioaba MA, Park JG, Guo Y, Tenaglia E, Xu C, Gish G, Min J, Pawson T. Proc Natl Acad Sci U S A. 2009 Dec 1;106(48):20336-41. doi: