PIWIL1

Gene Symbol PIWIL1
Entrez Gene 9271
Alt Symbol CT80.1, HIWI, MIWI, PIWI
Species Human
Gene Type protein-coding
Description piwi-like RNA-mediated gene silencing 1
Other Description piwi homolog|piwi-like protein 1
Swissprots Q96JD5 Q8TBY5 Q96J94 A4F266 Q8NA60 O95404
Accessions CBH19412 CBH30591 EAW98509 EAW98510 EAW98511 Q96J94 AB274731 BAF49084 AF104260 AAC97371 AF264004 AAK92281 AF387507 AAK69348 AK093133 BAC04068 BC028581 AAH28581 BG718140 DB072454 DB527532 JF432823 ADZ16040 XM_005253632 XP_005253689 XM_006719698 XP_006719761 XM_011539002 XP_011537304 XM_011539003 XP_011537305 XM_011539004 XP_011537306 XM_011539005 XP_011537307 XM_011539006 XP_011537308 XM_011546618 XP_011544920 XM_011546619 XP_011544921 XM_011546620 XP_011544922 XM_011546621 XP_011544923 XR_944838 XR_944839 NM_001190971 NP_001177900 NM_004764 NP_004755
Function Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative dev
Subcellular Location Cytoplasm {ECO:0000250}. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. Also present in chromatoid body (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in spermatocytes and spermatids. Also detected in prostate cancer (at protein level). Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. {ECO:0000269|PubMed:11154219, ECO:0000269|PubMed:12037681, ECO:0000269|PubMed:12906857, ECO:0000269|PubMed:23436708}.
Top Pathways Dorso-ventral axis formation

Miwi (G82) Antibody - 2079 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various mouse tissues using Miwi (G82) Antibody (upper panel) or β-Actin Antibody (#4967) (lower panel).

Miwi (D478) Antibody - 2025 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various mouse tissues using Miwi (D478) Antibody (upper panel) or β-Actin Antibody #4967 (lower panel).

PIWIL1 (N-17) - sc-22685 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIWIL1 (N-17): sc-22685. Western blot analysis of PIWIL1 expression in mouse testis (A) and rat testis (B) tissue extracts.

Anti-PIWIL1 antibody - ab12337 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Image courtesy of Human Protein Atlas ab12337 staining PIWI Human female colon, showing a distinct and strong staining pattern at glandular cells. Paraffin embedded human colon tissue was incubated with ab12337 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab12337 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.

Anti-PIWIL1 antibody - ab105393 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of PIWIL1 in rat liver tissue with ab105393 at 20 ug/mL.

Anti-PIWIL1 antibody - ab85125 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PIWIL1 antibody (ab85125) at 1/1200 dilution + Human testis lysate

Anti-PIWIL1 antibody - ab76692 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab76692 at 1/10 dilution, staining PIWIL1 in human brain by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PIWIL1 antibody - ab89427 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Anti-PIWIL1 antibody (ab89427) at 1 µg/ml + HepG2 cell lysate at 50 µg

Anti-PIWIL1 antibody [EPR13812(B)] - ab181056 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR13812(B)
All lanes : Anti-PIWIL1 antibody [EPR13812(B)] (ab181056) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : HepG2 cell lysateLane 3 : Human testis lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

Anti-PIWIL1 antibody - N-terminal - ab76693 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab76693 at 1/10 dilution, staining PIWIL1 in human testis by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-PIWIL1 / PIWI Antibody - LS-C91923 from LifeSpan Bioscience

Prices $295.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal

Anti-PIWIL1 / PIWI Antibody (aa562-574) - LS-C55292 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µg
Host Goat
Clonality Polyclonal

Anti-PIWIL1 / PIWI Antibody (aa82-290, clone 12K163) - LS-C182931 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 12K163

Anti-PIWIL1 / PIWI Antibody - LS-C51669 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
Western blot analysis: Lane 1: EasyWestern Protein Standard Lane 2: Mouse testis lysate Primary antibody: 1 ug/ml Rabbit Anti-Piwi-like Protein 1 Polyclonal Antibody LS-C51669 Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody

Anti-PIWIL1 / PIWI Antibody (C-Terminus) - LS-C110630 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIWIL1 antibody LS-C110630 Western blot of CCRF-CEM lysate.

Anti-PIWIL1 / PIWI Antibody (aa71-120) - LS-C110629 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIWIL1 antibody LS-C110629 Western blot of Fetal Stomach lysate.

Anti-PIWIL1 / PIWI Antibody (Internal) - LS-C136918 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PIWIL1 / PIWI Antibody - LS-C193077 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of extracts of various cell lines, using MIWI antibody.

Anti-PIWIL1 / PIWI Antibody - LS-C335209 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of various cell lines, using MIWI antibody.

Anti-PIWIL1 / PIWI Antibody - LS-C136956 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PIWIL1 / PIWI Antibody (N-Terminus) - LS-C115922 from LifeSpan Bioscience

Prices $345.00, $1,035.00
Sizes 100 µg, 500 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescence of PIWI-L1 in rat liver tissue with PIWI-L1 antibody at 20 ug/ml.

PIWIL1/HIWI Antibody - AF6548 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal

PIWIL1/HIWI Antibody (585509) - MAB6548 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 585509
PIWIL1/HIWI was detected in immersion fixed NCI‑N87 human gastric carcinoma cell line using Mouse Anti-Human PIWIL1/HIWI Monoclonal Antibody (Catalog # MAB6548) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm in colonies of NCI-N87 cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PIWIL1/HIWI Antibody - NBP1-54796 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PIWIL1/HIWI Antibody - NBP1-55299 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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