ICC/IF image of ab87939 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-PIWIL4 antibody (ab87939) at 1/500 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : Human fetal testis cell lysate
ab87939 at 1/100 dilution, staining PIWIL4 in formalin-fixed paraffin-embedded testis tissue showing nuclear staining.