PIWIL4

Gene Symbol PIWIL4
Entrez Gene 143689
Alt Symbol HIWI2, MIWI2
Species Human
Gene Type protein-coding
Description piwi-like RNA-mediated gene silencing 4
Other Description piwi-like 4|piwi-like protein 4
Swissprots Q8N9V8 Q68CZ4 B4DEG5 Q8NEH2 Q8N8G9 Q7Z3Z4
Accessions EAW66940 EAW66941 Q7Z3Z4 AB079366 BAC81341 AF086344 AK093475 BAC04179 AK096837 BAC04873 AK293616 BAG57076 BC031060 AAH31060 CR749642 CAH18436 DB091271 DQ891240 ABM82166 DQ894425 ABM85351 NM_152431 NP_689644
Function Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromat
Subcellular Location Nucleus {ECO:0000250}. Cytoplasm {ECO:0000250}. Note=Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. PIWIL2/MILI is required for nuclear localization (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed. {ECO:0000269|PubMed:17544373}.
Top Pathways Dorso-ventral axis formation

PIWIL4 (H-90) - sc-68932 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PIWIL4 (H-90): sc-68932. Western blot analysis of PIWIL4 expression in NTERA-2 cl.D1 whole cell lysate.

PIWIL4 (M-80) - sc-68933 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

PIWIL4 (Y-18) - sc-67593 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PIWIL4 (Y-18): sc-67593. Western blot analysis of PIWIL4 expression in NTERA-2 cl.D1 (A), WI 38 (B) and PC-3 (C) whole cell lysates.

PIWIL4 (T-16) - sc-67591 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-PIWIL4 antibody - ab21869 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : Anti-PIWIL4 antibody (ab21869) at 2 µg/mlLanes 3 - 4 : Anti-PIWIL4 antibody (ab21869) at 1 µg/mlLane 1 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)Lane 2 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/mlLane 3 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027)Lane 4 : Testis (Mouse) Tissue Lysate - normal tissue (ab4027) with Mouse PIWIL4 peptide (ab23535) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryRabbit IgG secondary antibody (ab28446) at 1/10000 dilutionPerformed under reducing conditions.

Anti-PIWIL4 antibody - ab180867 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab180867. Blue DAPI for nuclear staining.

Anti-PIWIL4 antibody - ab111714 from Abcam

Prices $384.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-PIWIL4 antibody (ab111714) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

Anti-PIWIL4 antibody - ab87939 from Abcam

Host Rabbit
Clonality Polyclonal
ICC/IF image of ab87939 stained HeLa cells. The cells were 4% Formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab87939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 Goat anti-Rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PIWIL4 antibody - ab189356 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human Testis, Spermatogonia tissue labeling PIWIL4 with ab189356 at 10 µg/ml.

Anti-PIWIL4 antibody - ab182760 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PIWIL4 antibody (ab182760) at 1/550 dilutionLane 1 : HeLa cell lysateLane 2 : 231 cell lysateLane 3 : 293T cell lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique

Anti-PIWIL4 antibody - ab168200 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-PIWIL4 antibody (ab168200) at 1 µg/mlLane 1 : PIWIL4 transfected 293T cell lysate Lane 2 : Non-transfected 293T cell lysate Lysates/proteins at 15 µl per lane.SecondaryGoat Anti-Mouse IgG developed using the ECL technique

Anti-PIWIL4 antibody - ab104345 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Anti-PIWIL4 antibody (ab104345) at 1 µg/ml + HT1080 cell lysate at 10 µg

Anti-HIWI2 / PIWIL4 Antibody (aa304-434, clone 10G9B11) - LS-C191806 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10G9B11
IHC of paraffin-embedded ovarian cancer tissues using PIWIL4 mouse monoclonal antibody with DAB staining.

Anti-HIWI2 / PIWIL4 Antibody (aa304-434, clone 10G9B11) - LS-C191807 from LifeSpan Bioscience

Prices $365.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 10G9B11
IHC of paraffin-embedded ovarian cancer tissues using PIWIL4 mouse monoclonal antibody with DAB staining.

Anti-HIWI2 / PIWIL4 Antibody - LS-C341553 from LifeSpan Bioscience

Prices $385.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-HIWI2 / PIWIL4 Antibody (aa50-255) IHC-plus™ - LS-B8707 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-HIWI2 / PIWIL4 antibody IHC staining of human testis, spermatogonia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-HIWI2 / PIWIL4 Antibody (N-Terminus) - LS-C111448 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIWIL4 antibody LS-C111448 Western blot of Fetal Brain lysate.

Anti-HIWI2 / PIWIL4 Antibody (aa35-84) - LS-C116524 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PIWIL4 antibody LS-C116524 Western blot of HT1080 cell lysate.

Anti-HIWI2 / PIWIL4 Antibody - LS-C136942 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-HIWI2 / PIWIL4 Antibody - LS-C335299 from LifeSpan Bioscience

Prices $255.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry of paraffin-embedded rat testis tissue using PIWIL4 antibody at dilution of 1:200 (x400 lens).

PIWIL4 Antibody (10G9B11) - NBP2-37398 from Novus Biologicals

Prices $369.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 10G9B11
Western Blot: PIWIL4 Antibody (10G9B11) [NBP2-37398] - Western blot analysis using PIWIL4 mouse mAb against PC-3 (1) and PANC-1 (2) cell lysate.

PIWIL4 Antibody (626001) - MAB6316 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 626001
PIWIL4 was detected in immersion fixed BG01V human embryonic stem cells using Human PIWIL4 Monoclonal Antibody (Catalog # MAB6316) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

PIWIL4 Antibody - NBP2-19845 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: PIWIL4 Antibody [NBP2-19845] - Sample (30 ug of whole cell lysate) A: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.

PIWIL4 Antibody - NBP1-83491 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemistry/Immunofluorescence: PIWIL4 Antibody [NBP1-83491] - Staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli, cytoplasm and golgi apparatus.

PIWIL4 Antibody - NBP1-54343 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
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