Overlay histogram showing SHSY-5Y cells stained with ab75996 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75996, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
![All lanes : Anti-PKA 2 beta (regulatory subunit) antibody [EP2648] (ab75996) at 1/20000 dilutionLane 1 : Fetal brain lysateLane 2 : Human testis lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12363_ab75996_1.JPG)
All lanes : Anti-PKA 2 beta (regulatory subunit) antibody [EP2648] (ab75996) at 1/20000 dilutionLane 1 : Fetal brain lysateLane 2 : Human testis lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution
ab75996, at 1/100 dilution, staining PKA 2 beta (regulatory subunit) in paraffin-embedded human brain tissue by Immunohistochemistry.