Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y]
| Name | Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab75993 |
| Prices | $387.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP2649Y |
| Applications | WB IP IHC-P FC ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to residues near the C terminus of human PKA 2 beta (regulatory subunit) |
| Description | Rabbit Monoclonal |
| Gene | PRKAR2B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) at 1/50000 dilution + Human brain lysate at 10 µgSecondarygoat anti rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12365_ab75993_WB_1.jpg)
Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) at 1/50000 dilution + Human brain lysate at 10 µgSecondarygoat anti rabbit HRP at 1/2000 dilution
ab75993, at a 1/100 dilution, staining PKA 2 beta (regulatory subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
Overlay histogram showing SH-SY5Y cells stained with ab75993 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75993, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.