Anti-PKA R2 antibody
| Name | Anti-PKA R2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab38949 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IP |
| Species Reactivities | Mouse, Rat, Rabbit, Human |
| Antigen | Synthetic peptide of Rat cAMP-dependent protein kinase type II-alpha regulatory subunit |
| Description | Rabbit Polyclonal |
| Gene | PRKAR2A |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab38949 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ICC/IF image of ab38949 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
PKA signaling drives mammary tumorigenesis through Src. - PKA signaling drives mammary tumorigenesis through Src.
Beristain AG, Molyneux SD, Joshi PA, Pomroy NC, Di Grappa MA, Chang MC, Kirschner LS, Prive GG, Pujana MA, Khokha R. Oncogene. 2015 Feb 26;34(9):1160-73.
beta-adrenergic signaling inhibits Gq-dependent protein kinase D activation by - beta-adrenergic signaling inhibits Gq-dependent protein kinase D activation by
Nichols CB, Chang CW, Ferrero M, Wood BM, Stein ML, Ferguson AJ, Ha D, Rigor RR, Bossuyt S, Bossuyt J. Circ Res. 2014 Apr 25;114(9):1398-409.
Cdkn2a/p16Ink4a regulates fasting-induced hepatic gluconeogenesis through the - Cdkn2a/p16Ink4a regulates fasting-induced hepatic gluconeogenesis through the
Bantubungi K, Hannou SA, Caron-Houde S, Vallez E, Baron M, Lucas A, Bouchaert E, Paumelle R, Tailleux A, Staels B. Diabetes. 2014 Oct;63(10):3199-209.