Anti-PKC alpha (phospho S657 + Y658) antibody
| Name | Anti-PKC alpha (phospho S657 + Y658) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab23513 |
| Prices | $400.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB ELISA IP IHC-P |
| Species Reactivities | Mouse, Rat, Rabbit, Human |
| Antigen | Phospho-PKC alpha (Ser-657/Tyr-658) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around serine 657 and tyrosine 658 of human PKC alpha |
| Blocking Peptide | Human PKC alpha (phospho S657 + Y658) peptide |
| Description | Rabbit Polyclonal |
| Gene | PRKCA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKCα (Ser-657/Tyr-658) (PP1091) antibody in the absence (Left) or presence (Right) of blocking peptide (PX1095). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594
Immunofluorescence analysis of S2-013 cells, staining PKC alpha (phospho S657 + Y658) with ab23513. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with blocking solution (3% BSA/PBS). Cells were incubated with primary antibody for 1 hour. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.
ab23513 staining PKC alpha (phospho S657 + Y658) in Human pancreatic ductal adenocarcinoma tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with PBS + Triton 0.025% and blocked with 5% serum for 1 hour ta room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.See Abreview
Product References
Fluorosed mouse ameloblasts have increased SATB1 retention and Galphaq activity. - Fluorosed mouse ameloblasts have increased SATB1 retention and Galphaq activity.
Zhang Y, Kim JY, Horst O, Nakano Y, Zhu L, Radlanski RJ, Ho S, Den Besten PK. PLoS One. 2014 Aug 4;9(8):e103994.
BART inhibits pancreatic cancer cell invasion by PKCalpha inactivation through - BART inhibits pancreatic cancer cell invasion by PKCalpha inactivation through
Taniuchi K, Yokotani K, Saibara T. PLoS One. 2012;7(4):e35674.
Changes in microvascular reactivity after cardiopulmonary bypass in patients with - Changes in microvascular reactivity after cardiopulmonary bypass in patients with
Feng J, Liu Y, Chu LM, Singh AK, Dobrilovic N, Fingleton JG, Clements RT, Bianchi C, Sellke FW. Circulation. 2012 Sep 11;126(11 Suppl 1):S73-80.
Phospholipase C and protein kinase C-beta 2 mediate insulin-like growth factor - Phospholipase C and protein kinase C-beta 2 mediate insulin-like growth factor
El-Shewy HM, Abdel-Samie SA, Al Qalam AM, Lee MH, Kitatani K, Anelli V, Jaffa AA, Obeid LM, Luttrell LM. Mol Endocrinol. 2011 Dec;25(12):2144-56.