PRKCA

Gene Symbol PRKCA
Entrez Gene 5578
Alt Symbol AAG6, PKC-alpha, PKCA, PRKACA
Species Human
Gene Type protein-coding
Description protein kinase C, alpha
Other Description PKC-A|aging-associated gene 6|protein kinase C alpha type
Swissprots Q96RE4 P17252 B5BU22 Q15137 Q32M72
Accessions AAK84184 ABY87554 AGC09604 CAJ00128 CAV30668 EAW89014 P17252 AA017296 AB209475 BAD92712 AB451258 BAG70072 AB451383 BAG70197 AF035594 AF035595 AF086287 AK055431 AK125425 AK130736 AL596674 AY633609 AAV33302 BC015855 BC053321 AAH53321 BC062759 BC071767 BC103691 BC107592 BC109273 AAI09274 BC109274 AAI09275 BC122530 BM015483 BQ018414 BU150006 BX648954 CA417000 EU832768 ACE87779 GQ129311 ACT64445 M22199 AAA60098 X52479 CAA36718 XM_011524989 XP_011523291 XM_011524990 XP_011523292 XM_011524991 XP_011523293 XM_011524992 XP_011523294 NM_002737 NP_002728
Function Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells. In intestinal cells stimulated by the phorbol ester PMA, can trigger a cell cycle arrest program which is associated with the accumulation of the hyper-phosphorylated growth-suppr
Subcellular Location Cytoplasm. Cell membrane; Peripheral membrane protein. Mitochondrion membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Nucleus.
Top Pathways Wnt signaling pathway, Tight junction, VEGF signaling pathway, Focal adhesion, Gap junction

Phospho-PKC (pan) (ζ Thr410) (190D10) Rabbit mAb (Biotinylated) - 5016 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone 190D10

Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb - 2060 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 190D10
Western blot analysis of baculovirus-expressed PKC isoforms, using Phospho-PKC (pan) (zeta Thr410) (190D10) Rabbit mAb.

Phospho-PKC (pan) (βII Ser660) Antibody - 9371 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of Baculovirus expressed PKCβ and PKCβ Ser660/Ala mutant, using Phospho-PKC (pan) (βII Ser660) Antibody (upper) or control PKCβ antibody (lower).

Phospho-PKCα/β II (Thr638/641) Antibody - 9375 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of Baculovirus-expressed PKC isoforms alpha, beta, gamma, delta and epsilon, untreated or treated with lambda protein phosphatase, using Phospho-PKCalpha/beta II (Thr638/641) Antibody (upper) or PKCalpha, beta, gamma, delta, epsilon antibodies (lower).

Phospho-PKC (pan) (gamma Thr514) Antibody - 9379 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from U937, C6 and HeLa cells, untreated, TPA-treated, or lambda phosphatase-treated as indicated, using Phospho-PKC (pan) (gamma Thr514) Antibody.

PKCα Antibody - 2056 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of HeLa, COS, C6 and NIH/3T3 cells, using PKCα Antibody.

PKC (A-3) - sc-17769 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC (A-3): sc-17769. Western blot analysis of PKC expression in K-562 (A), NIH/3T3 (B) and 3611-RF (C) whole cell lysates.

p-PKC α (A-11) - sc-377565 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of PKC α phosphorylation in untreated (A,D),Ser/Thr Phosphorylation Induction Cocktail (sc-362324) treated (B,E) and Ser/Thr Phosphorylation Induction Cocktail (sc-362324) and lambda protein phosphatase (sc-200312A) treated (C,F) Jurkat whole cell lysates. Antibodies tested include p-PKC α (A-11): sc-377565 (A,B,C) and PKC α (H-7): sc-8393 (D,E,F).

PKC (A-9) - sc-17804 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC (A-9): sc-17804. Western blot analysis of PKC expression in HeLa (A), Jurkat (B), NIH/3T3 (C) and 3611-RF (D) whole cell lysates.

PKC (H-300) - sc-10800 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC (H-300): sc-10800. Immunofluorescence staining of normal mouse liver frozen section showing membrane and cytoplasmic staining.

PKC (MC5) - sc-80 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of l phosphatase-treated (A,C) and untreated (B,D) active human recombinant PKCα. Antibodies tested include PKC (MC5): sc-80 (A,B) and p-PKCα (Ser 657): sc-12356 (C,D).

PKC α (C-20) - sc-208 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC α (C-20)-G: sc-208-G. Western blot analysis of PKC α expression in Jurkat (A) and NIH/3T3 (B) whole cell lysates and rat brain extract (C).

PKC α (H-7) - sc-8393 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC α (H-7): sc-8393. Immunofluorescence staining of methanol-fixed K-562 cells showing cytoplasmic staining.

p-PKC α (Ser 657) - sc-12356 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Western blot detection of PKC a phosphorylation using HeLa nuclear extracts treated with PMA. Blots were probed with PKC a (H-7): sc-8393 (A) and PKC a (Ser 657)-R: sc-12356-R (B,C). Antibody was preincubated with cognate non-phosphorylated (B) or phosphorylated (C) peptide.

Anti-PKC (phospho T410) antibody - ab51166 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC (phospho T410) antibody (ab51166) at 1/500 dilutionLane 1 : Extracts from 3T3 cells treated with PMA (125ng/ml, 30min) plus phosphopeptide.Lane 2 : Extracts from 3T3 cells treated with PMA (125ng/ml, 30min).

Anti-PKC (phospho T497) antibody - ab59411 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue, using ab59411 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.

Anti-PKC (phospho T497) antibody - ab192196 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC (phospho T497) antibody (ab192196) at 1/500 dilutionLane 1 : NIH 3T3 whole cell lysate with Antigen-specific peptideLane 2 : NIH 3T3 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-PKC alpha + beta + gamma antibody [EPR16898] - ab179522 from Abcam

Prices $99.00, $357.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR16898
All lanes : Anti-PKC alpha + beta + gamma antibody [EPR16898] (ab179522) at 1/10000 dilutionLane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µgLane 2 : 293T (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µgLane 3 : SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Anti-PKC alpha + beta 2 (phospho T638) antibody - ab59365 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59365, at a 1/50 dilution, staining human PKC alpha + beta 2 in breast carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).

Anti-PKC alpha + beta 2 antibody - ab59328 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59328 at 1/50 dilution staining human breast carcinoma without (left) and with (right) immunizing peptide; paraffin embedded.

Anti-PKC alpha (phospho S657 + Y658) antibody - ab23513 from Abcam

Prices $400.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunocytochemical labeling of PKC phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-PKCα (Ser-657/Tyr-658) (PP1091) antibody in the absence (Left) or presence (Right) of blocking peptide (PX1095). The antibody was detected using appropriate secondary antibody conjugated to DyLight® 594

Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] - ab180848 from Abcam

Prices $99.00, $357.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1901(2)
All lanes : Anti-PKC alpha (phospho S657) antibody [EPR1901(2)] (ab180848)Lane 1 : Jurkat cell lysateLane 2 : Lysate from Jurkat cells treated with phorbol-12,13-dibutyrateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique

Anti-PKC alpha (phospho T497) antibody [EP2608Y] - ab76016 from Abcam

Prices $99.00, $387.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2608Y
All lanes : Anti-PKC alpha (phospho T497) antibody [EP2608Y] (ab76016) at 1/100000 dilutionLane 1 : Jurkat cell lysate, untreatedLane 2 : Jurkat cell lysate treated with LPLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilution

Anti-PKC alpha (phospho T638) antibody - ab195769 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC alpha (phospho T638) antibody (ab195769) at 1/500 dilutionLane 1 : UV treated NIH 3T3 whole cell lysates with antigen-specific peptideLane 2 : UV treated NIH 3T3 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-PKC alpha (phospho Y657) antibody - ab63386 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC alpha (phospho Y657) antibody (ab63386) at 1/500 dilutionLane 1 : COLO205 cell extractLane 2 : COLO205 cell extract with immunising phosphopeptide at 10 µgLysates/proteins at 30 µg per lane.
Search more