Anti-PKC alpha antibody [Y124]
| Name | Anti-PKC alpha antibody [Y124] |
|---|---|
| Supplier | Abcam |
| Catalog | ab32376 |
| Prices | $99.00, $404.00 |
| Sizes | 10 µl, 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | Y124 |
| Applications | WB IHC-P ICC/IF ICC/IF FC IP IHC-F |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in C terminus of human PKC alpha |
| Blocking Peptide | PKC alpha peptide |
| Description | Rabbit Monoclonal |
| Gene | PRKCA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-PKC alpha antibody [Y124] (ab32376) at 1/10000 dilution + 293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12474_ab32376wb.bmp)
Anti-PKC alpha antibody [Y124] (ab32376) at 1/10000 dilution + 293 cell lysate
Ab32376, at a 1/250 dilution, staining PKC alpha in paraffin embedded human lung carcinoma tissue sections by Immunohistochemistry.
Ab32376, at a 1/250 dilution, staining PKC alpha in HeLa cells by Immunofluorescence.
See Abreview
Overlay histogram showing HeLa cells stained with ab32376 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32376, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Immunohistochemical analysis of mouse small intestine tissue, staining PKC alpha with ab32376.
Product References
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