
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab74007, at 1/50 dilution, in the presence (right panel) or absence (left panel) of the immunising phophopeptide.

All lanes : Anti-PKC delta (phospho Y52) antibody (ab74007) at 1/500 dilutionLane 1 : Extracts from Jurkat cells starved for 24hoursLane 2 : Extracts from Jurkat cells starved for 24hours with immunising phosphopeptide at 10 µgLysates/proteins at 15 µg per lane.

Immunofluorescence analysis of NIH/3T3 cells using ab74007, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.