All lanes : Anti-PKC epsilon antibody [1B7] (ab114020) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PKC epsilon cDNALysates/proteins at 5 µg per lane.
All lanes : Anti-PKC epsilon antibody [1B7] (ab114020) at 1/1000 dilutionLane 1 : HepG2 cell extractLane 2 : HeLa cell extractLane 3 : HT29 cell extractLane 4 : A549 cell extractLane 5 : COS7 cell extractLane 6 : Jurkat cell extractLane 7 : MDCK cell extractLane 8 : PC12 cell extractLane 9 : MCF7 cell extractLysates/proteins at 35 µg per lane.
ab114020, at 1/100 dilution, staining PKC epsilon in COS7 cells transiently transfected with pCMV6-ENTRY PKC epsilon by Immunofluorescence.
ab114020, at 1/150 dilution, staining PKC epsilon in paraffin-embedded Human thyroid tissue by Immunohistochemistry.
ab114020, at 1/150 dilution, staining PKC epsilon in paraffin-embedded Human prostate tissue by Immunohistochemistry.
ab114020, at 1/150 dilution, staining PKC epsilon in paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry.
Flow cytometric analysis of PKC epsilon in Hela cells, using ab114020 at 1/100 dilution (Red) compared to a nonspecific negative control antibody (Blue).