PRKCE

Gene Symbol PRKCE
Entrez Gene 5581
Alt Symbol PKCE, nPKC-epsilon
Species Human
Gene Type protein-coding
Description protein kinase C, epsilon
Other Description protein kinase C epsilon type
Swissprots Q9UE81 Q02156 Q53SM5 B0LPH7 Q53SL4 Q32MQ3
Accessions AAD08855 AAX93119 AAX93253 AAY14773 ABY87556 AGC09605 EAX00257 EAX00258 EAX00259 Q02156 AK128749 AK313842 BAG36574 AL832345 BC029576 BC051195 BC054052 BC109033 AAI09034 BC109034 AAI09035 CA424727 X65293 CAA46388 XM_005264428 XP_005264485 XM_005264429 XP_005264486 XM_005264431 XP_005264488 XM_006712050 XP_006712113 XM_011532970 XP_011531272 XM_011532971 XP_011531273 XM_011532972 XP_011531274 XM_011532973 XP_011531275 XM_011532974 XP_011531276 XM_011532975 XP_011531277 XM_011532976 XP_011531278 XM_011532977 XP_011531279 XM_011532978 XP_011531280 XM_011532979 XP_011531281 XM_011532980 XP_011531282 XM_011532981 XP_011531283 XM_011532982 XP_011531284 XM_011532983 XP_011531285 XR_939695 NM_005400 NP_005391
Function Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17603037}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:17603037}. Cell membrane {ECO:0000269|PubMed:17603037}. Cytoplasm, perinuclear region {ECO:0000250}. Nucleus {ECO:0000250}. Note=Translocated to plasma membrane in epithelial cells stimulated by HGF. Associated with the Golgi at the perinuclear site in pre-passage fibroblasts (By similarity). In passaging cells, translocated to the cell periphery (By similarity). Translocated to the nucleus in PMA- treated cells (By similarity). {ECO:0000250}.
Top Pathways Vascular smooth muscle contraction, Sphingolipid signaling pathway, cGMP-PKG signaling pathway, Inflammatory mediator regulation of TRP channels, MicroRNAs in cancer

PKCε (22B10) Rabbit mAb - 2683 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone 22B10
Western blot analysis of extracts from mouse brain, PC12 and COS cells using PKCε (22B10) Rabbit mAb.

PKC ε (C-15) - sc-214 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC ε (C-15)-G: sc-214-G. Western blot analysis of PKC ε expression in IMR-32 (A) and H4 (B) whole cell lysates and rat heart extract (C).

PKC ε (E-5) - sc-1681 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC ε (E-5): sc-1681. Western blot analysis of PKC ε expression in IMR-32 whole cell lysate.

PKC ε (17) - sc-56944 from Santa Cruz Biotechnology

Prices $279.00
Sizes 1000 µl supernatant
Host Mouse
Clonality Monoclonal
PKC ε (17): sc-56944. Western blot analysis of PKC ε expression in IMR-32 whole cell lysate (A) and mouse brain tissue extract (B).

p-PKC ε (Ser 729) - sc-12355 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-PKC ε (Ser 729)-R: sc-12355-R. Western blot analysis of p-PKC ε expression in HeLa (A) and PMA-treated HeLa (B) whole cell lysates.

Anti-PKC epsilon (phospho S729) antibody - ab63387 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC epsilon (phospho S729) antibody (ab63387) at 1/500 dilutionLane 1 : HeLa cells treated with PMA (125ng/ml, 30mins) at 30 µgLane 2 : Jurkat cells treated eith PMA (125ng/ml, 30mins) at 30 µgLane 3 : NIH/3T3 cells treated with PMA (125ng/ml, 30mins) at 30 µgLane 4 : NIH/3T3 cells treated with syntesized peptide (125ng/ml, 30mins) at 10 µg

Anti-PKC epsilon (phospho S729) antibody - ab88241 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC epsilon (phospho S729) antibody (ab88241) at 1 µg/mlLane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Lane 2 : NIH 3T3 treated with Vanadate and PDGF; Whole Cell Lysate Lane 3 : NIH 3T3 Whole Cell Lysate with Immunising Peptide at 1 µg/mlLane 4 : NIH 3T3 treated with Vanadate and PDGF; Whole Cell Lysate with Immunising Peptide at 1 µg/mlLane 5 : NIH 3T3 Whole Cell Lysate with Non- Modified Control Peptide at 1 µg/mlLane 6 : NIH 3T3 treated with Vanadate and PDGF; Whole Cell Lysate with Non- Modified Control Peptide at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PKC epsilon (phospho S729) antibody [EPR1483(N)] - Carrier Free - ab181119 from Abcam

Prices $99.00, $357.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1483(N)
All lanes : Anti-PKC epsilon (phospho S729) antibody [EPR1483(N)] - Carrier Free (ab181119) at 1/2000 dilutionLane 1 : Lysate of SH-SY5Y cells treated with Okadaic Acid and Calyculin ALane 2 : Lysate of SH-SY5Y cellsLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Anti-PKC epsilon antibody - ab63638 from Abcam

Prices $395.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab63638 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63638, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PKC epsilon antibody - ab4141 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PKC epsilon antibody - ab134031 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex neurons labelling PKC epsilon with ab134031 at 1/400 dilution. Followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-PKC epsilon antibody - ab54492 from Abcam

Prices $359.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PKC epsilon antibody [17] - ab11724 from Abcam

Prices $359.00
Sizes 1 ml
Host Mouse
Clonality Monoclonal
Clone 17

Anti-PKC epsilon antibody [1B4] - ab114034 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B4
All lanes : Anti-PKC epsilon antibody [1B4] (ab114034) at 1/500 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control cDNA Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PKC epsilon cDNA Lysates/proteins at 5 µg per lane.

Anti-PKC epsilon antibody [1B7] - ab114020 from Abcam

Prices $384.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1B7
All lanes : Anti-PKC epsilon antibody [1B7] (ab114020) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PKC epsilon cDNALysates/proteins at 5 µg per lane.

Anti-PKC epsilon antibody [EPR1482(2)] - ab124806 from Abcam

Prices $99.00, $388.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1482(2)
Anti-PKC epsilon antibody [EPR1482(2)] (ab124806) at 1/1000 dilution (unpurified) + Jurkat cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-PRKCE / PKC-Epsilon Antibody (C-Terminus) - LS-C26187 from LifeSpan Bioscience

Prices $475.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal

Anti-PRKCE / PKC-Epsilon Antibody (phospho-Ser729) - LS-C26185 from LifeSpan Bioscience

Prices $485.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCE / PKC-Epsilon Antibody (C-Terminus) - LS-C26190 from LifeSpan Bioscience

Prices $405.00
Sizes 1 ml
Host Rabbit
Clonality Polyclonal

Anti-PRKCE / PKC-Epsilon Antibody (aa721-737) - LS-C26189 from LifeSpan Bioscience

Prices $575.00
Sizes 2 ml
Host Mouse
Clonality Monoclonal

Anti-PRKCE / PKC-Epsilon Antibody (C-Terminus) - LS-C26191 from LifeSpan Bioscience

Prices $335.00
Sizes 1 µg
Host Rabbit
Clonality Polyclonal

Anti-PRKCE / PKC-Epsilon Antibody - LS-C38540 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCE / PKC-Epsilon Antibody (clone 22B10) - LS-C75178 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone 22B10

Anti-PRKCE / PKC-Epsilon Antibody (clone I27-K, FITC) - LS-C170832 from LifeSpan Bioscience

Prices $475.00
Sizes 100 tst
Host Rabbit
Clonality Polyclonal
Clone I27-K

Anti-PRKCE / PKC-Epsilon Antibody (aa726-737) - LS-C51542 from LifeSpan Bioscience

Prices $245.00
Sizes 40 µg
Host Rabbit
Clonality Polyclonal
Lane 1: Mouse brain lysate Lane 2: Rat brain lysate Primary Antibody: 1 ug/ml Rabbit Anti-PKC epsilon Polyclonal Antibody LS-C51542 Secondary antibody: Goat Anti-Rabbit IgG (H&L) [HRP] Polyclonal Antibody The signal was developed with LumiSensor HRP Substrate Kit
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