Anti-PKC epsilon antibody [EPR1482(2)]

• Supplier: Abcam
• Catalog: ab124806
• Prices: $99.00, $388.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR1482(2)
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PKC epsilon
• Description: Rabbit Monoclonal
• Gene: PRKCE
• Conjugate: Unconjugated

Product images

Anti-PKC epsilon antibody [EPR1482(2)] (ab124806) at 1/1000 dilution (unpurified) + Jurkat cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Anti-PKC epsilon antibody [EPR1482(2)] (ab124806) at 1/1500 dilution (purified) + Jurkat cell lysate at 10 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-PKC epsilon antibody [EPR1482(2)] (ab124806) at 1/1000 dilution (unpurified)Lane 1 : Mouse heart tissue lysateLane 2 : Rat heart tissue lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-PKC epsilon antibody [EPR1482(2)] (ab124806) at 1/1500 dilution (purified)Lane 1 : Mouse heart tissue lysateLane 2 : Rat heart tissue lysateLysates/proteins at 10 µg per lane.SecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

All lanes : Anti-PKC epsilon antibody [EPR1482(2)] (ab124806) at 1/10000 dilution (unpurified)Lane 1 : SH-SY5Y cell lysateLane 2 : MCF7 cell lysateLane 3 : HT29 cell lysateLane 4 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling PKC epsilon with unpurified ab124806 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling PKC epsilon with purified ab124806 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PKC epsilon with unpurified ab124806 at 1/100 dilution.

Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling PKC epsilon with purified ab124806 at 1/150. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.Control: primary antibody (1/150) and secondary antibody, ab150113, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

Unpurified ab124806 staining PKC epsilon in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/200 in PBS) for 16 hours at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.See Abreview

Overlay histogram showing SH-SY5Y cells stained with unpurified ab124806 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124806, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Flow cytometry analysis of SH-SY5Y cells labelling PKC epsilon with purified ab124806 (red) at 1/200. Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD

Product References

Targeting PKCepsilon by miR-143 regulates cell apoptosis in lung cancer. - Targeting PKCepsilon by miR-143 regulates cell apoptosis in lung cancer.

Zhang N, Su Y, Xu L. FEBS Lett. 2013 Nov 15;587(22):3661-7.