Anti-PKC iota (phospho T555 + T563) antibody
| Name | Anti-PKC iota (phospho T555 + T563) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab5813 |
| Prices | $382.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic phosphopeptide derived from a region of human PKC iota that contains threonine 555 |
| Description | Rabbit Polyclonal |
| Gene | PRKCI |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% low-fat milk-TBST buffer for one hour at room temperature, and incubated with ab5813 antibody for two hours at room temperature in a 3% low-fat milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the phosphopeptide corresponding to PKC iota [pT555] blocks the antibody signal. The peptide corresponding to PKC zeta [pT560] blocks the antibody signal and the peptides corresponding to P](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12655_ab5813_1.jpg)
Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% low-fat milk-TBST buffer for one hour at room temperature, and incubated with ab5813 antibody for two hours at room temperature in a 3% low-fat milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the phosphopeptide corresponding to PKC iota [pT555] blocks the antibody signal. The peptide corresponding to PKC zeta [pT560] blocks the antibody signal and the peptides corresponding to P
Product References
Clueless regulates aPKC activity and promotes self-renewal cell fate in - Clueless regulates aPKC activity and promotes self-renewal cell fate in
Goh LH, Zhou X, Lee MC, Lin S, Wang H, Luo Y, Yang X. Dev Biol. 2013 Sep 15;381(2):353-64.
Protein kinase C iota as a therapeutic target in alveolar rhabdomyosarcoma. - Protein kinase C iota as a therapeutic target in alveolar rhabdomyosarcoma.
Kikuchi K, Soundararajan A, Zarzabal LA, Weems CR, Nelon LD, Hampton ST, Michalek JE, Rubin BP, Fields AP, Keller C. Oncogene. 2013 Jan 17;32(3):286-95.
Aberrant expression of the polarity complex atypical PKC and non-muscle myosin - Aberrant expression of the polarity complex atypical PKC and non-muscle myosin
Wald FA, Forteza R, Diwadkar-Watkins R, Mashukova A, Duncan R, Abreu MT, Salas PJ. Virchows Arch. 2011 Sep;459(3):331-8.