PRKCI

Gene Symbol PRKCI
Entrez Gene 5584
Alt Symbol DXS1179E, PKCI, nPKC-iota
Species Human
Gene Type protein-coding
Description protein kinase C, iota
Other Description PRKC-lambda/iota|aPKC-lambda/iota|atypical protein kinase C-lambda/iota|protein kinase C iota type
Swissprots P41743 D3DNQ4 Q8WW06
Accessions EAW78513 EAW78514 EAW78515 P41743 AB451443 BAG70257 AI521422 AK315342 BAG37741 BC022016 AAH22016 BC042405 BM767350 BQ014217 CN412350 L18964 AAA60171 L33881 AAB17011 NM_002740 NP_002731
Function Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non- small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response
Subcellular Location Cytoplasm. Membrane. Endosome. Nucleus. Note=Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A.
Tissue Specificity Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers. {ECO:0000269|PubMed:15994303, ECO:0000269|PubMed:7607695, ECO:0000269|PubMed:8226978}.
Top Pathways Tight junction, Insulin signaling pathway, Endocytosis, Hippo signaling pathway, Rap1 signaling pathway

PKCι/λ (C83H11) Rabbit mAb - 2998 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C83H11
Western blot analysis of extracts from C2C12, PC12 and COS cells using PKCι/λ (C83H11) Rabbit mAb.

PKC λ/&iota (E-7) - sc-376344 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC λ/ι (E-7): sc-376344. Western blot analysis of PKC λ/ι expression in A-431 (A) and A549 (B) whole cell lysates.

PKC λ/ι (H-12) - sc-17837 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC λ/ι (H-12): sc-17837. Western blot analysis of PKC ι long form expression in NCI-H1688 (A), A549 (B), SHP-77 (C), A-431 (D), SK-N-MC (E) and H4 (F) whole cell lysates.

PKC λ/ι (H-20) - sc-32020 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKC λ/ι (H-20): sc-32020. Western blot analysis of PKC λ/ι expression in A-431 whole cell lysate.

PKC λ/ι (H-76) - sc-11399 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC λ/ι (H-76): sc-11399. Western blot analysis of PKC ι long and short forms expression in MIA PaCa-2 (A) and HeLa (B) whole cell lysates.

Anti-PKC iota (phospho T555 + T563) antibody - ab5813 from Abcam

Prices $382.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Peptide Competition and Phosphatase Treatment: Lysates prepared from Jurkat cells stimulated with PMA were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-4) or treated with lambda phosphatase (5), blocked with a 5% low-fat milk-TBST buffer for one hour at room temperature, and incubated with ab5813 antibody for two hours at room temperature in a 3% low-fat milk-TBST buffer, following prior incubation with: no peptide (1), the non-phosphopeptide corresponding to the immunogen (2), a generic phosphothreonine-containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignalTM method. The data show that the phosphopeptide corresponding to PKC iota [pT555] blocks the antibody signal. The peptide corresponding to PKC zeta [pT560] blocks the antibody signal and the peptides corresponding to P

Anti-PKC iota antibody - ab5282 from Abcam

Prices $384.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
ab5282 staining PKC iota in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PKC iota antibody - ab53878 from Abcam

Prices $370.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal

Anti-PKC iota antibody - ab57423 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-PKC iota antibody - ab57422 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57422 PKC iota antibody at 1ug/ml.

Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] - ab76129 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1491Y
Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Anti-PRKCI / PKC Iota Antibody (C-Terminus) - LS-C38541 from LifeSpan Bioscience

Prices $495.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus, AP) - LS-C281443 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus, FITC) - LS-C281446 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus) - LS-C209978 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (Internal) - LS-C209976 from LifeSpan Bioscience

Prices $295.00
Sizes 250 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus, Biotin) - LS-C281448 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus, APC) - LS-C281447 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus, Azide-free, HRP) - LS-C281449 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus, PE) - LS-C281450 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (N-Terminus) - LS-C330603 from LifeSpan Bioscience

Prices $425.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCI / PKC Iota Antibody (aa215-264) - LS-C117191 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
PRKCI / PKC-Iota antibody LS-C117191 Western blot of HepG2 cell lysate.

Anti-PRKCI / PKC Iota Antibody - LS-C331033 from LifeSpan Bioscience

Prices $305.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts of MCF7 cells tissue, using PRKCI antibody.

Anti-PRKCI / PKC Iota Antibody (aa176-207) - LS-C98927 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Anti-PRKCI / PKC Iota Antibody (clone 1G11) - LS-C197989 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 1G11
Immunofluorescence of monoclonal antibody to PRKCI on HeLa cell . [antibody concentration 10 ug/ml]
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