ab5282 staining PKC iota in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Anti-PKC iota antibody (ab5282) at 1/500 dilution + Active human PKC iota full length protein (ab60850) at 0.1 µgSecondaryActive rat MCP1 protein fragment (ab9780) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minute
Western blot analysis of ab5282 in mouse lung lysate. PKC iota (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.