All lanes : Anti-PKC mu (phospho S738 + S742) antibody (ab74105) at 1/500 dilutionLane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus phosphopeptide at 10 µg Lane 2 : Extracts from HT-29 cells treated with serum (20%, 15mins), plus phosphopeptide at 10 µg Lane 3 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus serum (20%, 15mins) and phopeptide at 10 µg Lysates/proteins at 5 µg per lane.
ab74105, at a 1/50 dilution, staining PKC mu in paraffin embedded human heart tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.