PRKD1

Gene Symbol PRKD1
Entrez Gene 5587
Alt Symbol PKC-MU, PKCM, PKD, PRKCM
Species Human
Gene Type protein-coding
Description protein kinase D1
Other Description nPKC-D1|nPKC-mu|protein kinase C mu type|protein kinase C, mu|protein kinase D|serine/threonine-protein kinase D1
Swissprots B2RAF6 A6NL64 Q15139
Accessions ABE96833 EAW65970 EAW65971 Q15139 AK314170 BAG36853 BC160015 AAI60015 BX645735 CN412379 X75756 CAA53384 XM_005267859 XP_005267916 XM_011536964 XP_011535266 XM_011536965 XP_011535267 XR_943493 NM_002742 NP_002733
Function Serine/threonine-protein kinase that converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, and is involved in the regulation of MAPK8/JNK1 and Ras signaling, Golgi membrane integrity and trafficking, cell survival through NF-kappa-B activation, cell migration, cell differentiation by mediating HDAC7 nuclear export, cell proliferation via MAPK1/3 (ERK1/2) signaling, and plays a role in cardiac hypertrophy, VEGFA-induced angiogenesis, genotoxic-induced apoptosis and flagellin-stimulated inflammatory response. Phosphorylates the epidermal growth factor receptor (EGFR) on dual threonine residues, which leads to the suppression of epidermal growth factor (EGF)-induced MAPK8/JNK1 activation and subsequent JUN phosphorylation. Phosphorylates RIN1, inducing RIN1 binding to 14-3-3 proteins YWHAB, YWHAE and YWHAZ and increased competition with RAF1 for binding to GTP-bound form of Ras proteins (NRAS, HRAS and KRAS). Acts downstream of the heterot
Subcellular Location Cytoplasm {ECO:0000269|PubMed:18076381}. Cell membrane {ECO:0000269|PubMed:18076381}. Golgi apparatus, trans-Golgi network {ECO:0000250}. Note=Translocation to the cell membrane is required for kinase activation.
Top Pathways Rap1 signaling pathway

Phospho-PKD/PKCμ (Ser744/748) Antibody - 2054 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using Phospho-PKD/PKCµ (Ser744/748) Antibody (upper) or PKD/PKCµ Antibody #2052 (lower).

Phospho-PKD/PKCμ (Ser916) Antibody - 2051 from Cell Signaling Technology

Prices $109.00, $287.00, $678.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots), 300 µl (30 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using Phospho-PKD/PKCµ (Ser916) Antibody (upper) or PKD/PKCµ Antibody #2052 (lower).

PKD/PKCμ Antibody - 2052 from Cell Signaling Technology

Prices $129.00, $239.00
Sizes 40 µl (8 western blots), 100 µl (20 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from NIH/3T3 cells, untreated, PDGF-treated (50 ng/ml) or TPA-treated (0.2 µM), using PKD/PKCmu Antibody.

PKC μ (C-20) - sc-639 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC μ (C-20): sc-639. Western blot analysis of PKC μ expression in K-562 whole cell lysate.

PKC μ (H-73) - sc-292444 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC μ (H-73): sc-292444. Western blot analysis of PKC μ expression in HeLa (A), K-562 (B), Jurkat (C) and THP-1 (D) whole cell lysates.

PKC μ (D-20) - sc-935 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC μ (D-20): sc-935. Western blot analysis of PKC μ expression in NIH/3T3 (A), 3611-RF (B), A-431 (C), HeLa (D), Jurkat (E) and K-562 (F) whole cell lysates.

p-PKC μ (Ser 738) - sc-101781 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKC μ (Ser 738): sc-101781. Western blot analysis of phosphorylated PKC μ expression in untreated (A) and EGF-treated (B) A-431 whole cell lysates.

p-PKC μ (Ser 910) - sc-101782 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKC μ (Ser 910): sc-101782. Western blot analysis of phosphorylated PKC μ expression in untreated (A) and EGF-treated (B) A431 whole cell lysates.

Anti-PKC mu (phospho S205) antibody - ab59413 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59413 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.

Anti-PKC mu (phospho S738 + S742) antibody - ab74105 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC mu (phospho S738 + S742) antibody (ab74105) at 1/500 dilutionLane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus phosphopeptide at 10 µg Lane 2 : Extracts from HT-29 cells treated with serum (20%, 15mins), plus phosphopeptide at 10 µg Lane 3 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus serum (20%, 15mins) and phopeptide at 10 µg Lysates/proteins at 5 µg per lane.

Anti-PKC mu (phospho S738 + S742) antibody [EP1493-62] - ab75878 from Abcam

Prices $99.00, $313.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1493-62
All lanes : Anti-PKC mu (phospho S738 + S742) antibody [EP1493-62] (ab75878) at 1/500 dilutionLane 1 : HeLa cell lysateLane 2 : HeLa cell lysate treated with TPALysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/1000 dilution

Anti-PKC mu (phospho S738) antibody - ab131101 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC mu (phospho S738) antibody (ab131101) at 1/500 dilutionLane 1 : MCF cell extract untreatedLane 2 : MCF cell extract treated with PMA

Anti-PKC mu (phospho S910) antibody - ab131267 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC mu (phospho S910) antibody (ab131267) at 1/500 dilutionLane 1 : 293 cell extract treated with EGFLane 2 : 293 cell extract untreatedLane 3 : HUVEC cell extract treated with PMALane 4 : HUVEC cell extract untreated

Anti-PKC mu (phospho S910) antibody - ab61787 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC mu (phospho S910) antibody (ab61787) at 1/500 dilutionLane 1 : Extracts from A431 cellsLane 2 : Extracts from A431 cells with immunising phosphopeptide

Anti-PKC mu (phospho S916) antibody [EP1494Y] - ab81218 from Abcam

Prices $99.00, $387.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1494Y
developed using the ECL techniquePerformed under reducing conditions.

Anti-PKC mu (phospho Y463) antibody - ab59415 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59415 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.

Anti-PKC mu antibody - ab5283 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-PKC mu antibody (ab5283) at 1/500 dilution + Active human PKC mu full length protein (ab60873) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 90 seconds

Anti-PKC mu antibody - ab131460 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC mu antibody (ab131460) at 1/500 dilutionLane 1 : HeLa cell extractLane 2 : 293 cell extractLane 3 : THP1 cell extractLane 4 : Jurkat cell extract

Anti-PKC mu antibody - ab172096 from Abcam

Prices $376.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Immunofluorescence of ab172096 on HeLa cell. [antibody concentration 10 ug/ml]

Anti-PKC mu antibody - ab59334 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC mu antibody (ab59334) at 1/500 dilutionLane 1 : extracts from Jurkat cellsLane 2 : extracts from Jurkat cells with immunizing peptide

Anti-PKC mu antibody - ab59366 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab59366, at a 1/50 dilution, staining human PKC mu in brain, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).

Anti-PKC mu antibody - ab59367 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab59367 at a 1/50 dilution.Left image un-treated.Right image treated with immunizing peptide.

Anti-PKC mu antibody - ab61124 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab61124 at 1/500 dilution immunofluorescent staining PKC mu in HeLa cells, treated with EGF (200nM, 5mins; left image). Staining of untreated cells in right image.

Anti-PKC mu antibody [EP1493Y] - ab51246 from Abcam

Prices $99.00, $379.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1493Y
All lanes : Anti-PKC mu antibody [EP1493Y] (ab51246) at 1/1000 dilutionLane 1 : HeLa cell lysate, untreatedLane 2 : HeLa cell lysate, treated with PMALysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP labeled at 1/2000 dilution

Anti-PKC mu antibody [EPR1492(2)] - ab108963 from Abcam

Prices $99.00, $381.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR1492(2)
All lanes : Anti-PKC mu antibody [EPR1492(2)] (ab108963) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : Jurkat cell lysateLane 3 : HepG2 cell lysateLane 4 : K562 cell lysateLysates/proteins at 10 µg per lane.
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