Anti-PKC mu antibody (ab5283) at 1/500 dilution + Active human PKC mu full length protein (ab60873) at 0.1 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 90 seconds
Western blot analysis of ab5283 in mouse kidney lysate. PKC mu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
ab5283 staining PKC mu in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).