Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y]

• Supplier: Abcam
• Catalog: ab76129
• Prices: $313.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1491Y
• Applications: IHC-P WB
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to PKC zeta
• Description: Rabbit Monoclonal
• Gene: PRKCI
• Conjugate: Unconjugated

Product images

Immunohistochemical staining of paraffin embedded rat cerebral cortex with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded mouse kidney with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Immunohistochemical staining of paraffin embedded human endometrium with purified ab76129 at a working dilution of 1 in 100. The secondary antibody used is a HRP goat anti-rabbit IgG (ab97051), used at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified) + Mouse lung tissue lysate at 10 µgSecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

All lanes : Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/20000 dilution (purified)Lane 1 : Untreated HeLa cellsLane 2 : HeLa cells treated with alkaline phosphataseLysates/proteins at 10 µg per lane.SecondaryHRP goat anti-rabbit (H+L) at 1/1000 dilution

Immunohistochemical staining of PKC zeta in human brain with unpurified ab76129 at 1/100 dilution.

All lanes : Anti-PKC zeta (phospho T410) + PKC lambda (phospho T412) antibody [EP1491Y] (ab76129) at 1/2000 dilution (unpurified)Lane 1 : 293 cell lysates, untreatedLane 2 : 293 cell lysates, treated with APLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit-HRP at 1/1000 dilution

Product References

MicroRNAs overexpressed in growth-restricted rat skeletal muscles regulate the - MicroRNAs overexpressed in growth-restricted rat skeletal muscles regulate the

Raychaudhuri S. PLoS One. 2012;7(4):e34596.