Anti-PKC zeta (phospho T560) antibody [EP2037AY]
| Name | Anti-PKC zeta (phospho T560) antibody [EP2037AY] |
|---|---|
| Supplier | Abcam |
| Catalog | ab62372 |
| Prices | $99.00, $390.00 |
| Sizes | 10 µl, 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP2037AY |
| Applications | WB IHC-P ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic phosphopeptide corresponding to residues surrounding Threonine 560 of human PKC zeta |
| Description | Rabbit Monoclonal |
| Gene | PRKCZ |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)Lane 1 : Hela Cell Lysate (untreated with Calyculin A)Lane 2 : Hela Cell Lysate (Treated with Calyculin A)](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/12738_PKC-zeta-Primary-antibodies-ab62372-1.jpg)
All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)Lane 1 : Hela Cell Lysate (untreated with Calyculin A)Lane 2 : Hela Cell Lysate (Treated with Calyculin A)
ab62372 at 1/100 dilution staining PKC zeta in human brain by Immunohistochemistry, Paraffin-embedded tissue.
ab62372 at 1/100 dilution staining PKC zeta in human breast ductal carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Overlay histogram showing HEK293 cells stained with ab62372 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab62372, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG; H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Targeting aPKC disables oncogenic signaling by both the EGFR and the - Targeting aPKC disables oncogenic signaling by both the EGFR and the
Kusne Y, Carrera-Silva EA, Perry AS, Rushing EJ, Mandell EK, Dietrich JD, Errasti AE, Gibbs D, Berens ME, Loftus JC, Hulme C, Yang W, Lu Z, Aldape K, Sanai N, Rothlin CV, Ghosh S. Sci Signal. 2014 Aug 12;7(338):ra75.
DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for - DNAX-activating protein 10 (DAP10) membrane adaptor associates with receptor for
Sakaguchi M, Murata H, Aoyama Y, Hibino T, Putranto EW, Ruma IM, Inoue Y, Sakaguchi Y, Yamamoto K, Kinoshita R, Futami J, Kataoka K, Iwatsuki K, Huh NH. J Biol Chem. 2014 Aug 22;289(34):23389-402.
Metabotropic actions of the volatile anaesthetic sevoflurane increase protein - Metabotropic actions of the volatile anaesthetic sevoflurane increase protein
Wang J, Meng F, Cottrell JE, Sacktor TC, Kass IS. J Physiol. 2012 Aug 15;590(Pt 16):4093-107.
PKCzeta mediates disturbed flow-induced endothelial apoptosis via p53 - PKCzeta mediates disturbed flow-induced endothelial apoptosis via p53
Heo KS, Lee H, Nigro P, Thomas T, Le NT, Chang E, McClain C, Reinhart-King CA, King MR, Berk BC, Fujiwara K, Woo CH, Abe J. J Cell Biol. 2011 May 30;193(5):867-84.