PRKCZ

Gene Symbol PRKCZ
Entrez Gene 5590
Alt Symbol PKC-ZETA, PKC2
Species Human
Gene Type protein-coding
Description protein kinase C, zeta
Other Description nPKC-zeta|protein kinase C zeta type
Swissprots A8K4N0 A8MU64 Q05513 B7Z2J7 Q15207 Q5SYT5 Q969S4 E9PCW2
Accessions EAW56130 EAW56131 Q05513 AB007974 AK097627 BAG53497 AK131526 AK290995 BAF83684 AK294649 BAH11833 AK294782 BAH11883 AK302160 BAH13641 AK310372 AL542576 AU134793 BC008058 AAH08058 BC014270 AAH14270 BT007082 AAP35745 CB152283 DC312466 DQ892929 ABM83855 DQ893922 ABM84848 DQ896178 ABM87177 L14283 AAA36488 Z15108 CAA78813 XM_011541773 XP_011540075 XM_011541774 XP_011540076 XM_011541775 XP_011540077 XM_011541776 XP_011540078 XM_011541777 XP_011540079 XM_011541778 XP_011540080 XM_011541779 XP_011540081 XM_011541780 XP_011540082 XR_946707 XR_946708 NM_001033581 NP_001028753 NM_001033582 NP_001028754 NM_001242874 NP_001229803 NM_002744 NP_002735
Function Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN p
Subcellular Location Cytoplasm. Endosome. Cell junction. Note=In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. {ECO:0000250}.
Tissue Specificity Expressed in brain, and to a lesser extent in lung, kidney and testis.
Top Pathways Chemokine signaling pathway, Sphingolipid signaling pathway, Rap1 signaling pathway, Hippo signaling pathway, PI3K-Akt signaling pathway

PKCζ (C24E6) Rabbit mAb - 9368 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C24E6
Western blot analysis of extracts from 293, NBT-II, PC12 and COS cells, using PKCζ (C24E6) Rabbit mAb.

PKCζ Antibody - 9372 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of Baculovirus expressed PKC isoforms, demonstrating the isoform-specificty of PKCζ Antibody.

PKC ζ (C-20) - sc-216 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKC ζ (C-20): sc-216. Western blot analysis of PKC ζ expression in NIH/3T3 (A), 3611-RF (B) and A-431 (C) whole cell lysates.

PKC ζ (H-1) - sc-17781 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKC δ (H-1): sc-17781. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

PKC ζ (N-17) - sc-7262 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKC ζ (N-17): sc-7262. Western blot analysis of PKC ζ expression in 3611-RF whole cell lysate.

p-PKC ζ (Thr 410) - sc-101778 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-PKC ζ (Thr 410): sc-101778. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung carcinoma tissue showing cytoplasmic staining.

Anti-PKC zeta (phospho T410) antibody - ab194922 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling PKC zeta (phospho T410) with ab194922 at 1/50 dilution.

Anti-PKC zeta (phospho T560) antibody - ab59412 from Abcam

Prices $390.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC zeta (phospho T560) antibody (ab59412) at 1/500 dilutionLane 1 : Extracts from COS7 cells treated with PMA (125ng/ml, 30mins)Lane 2 : Extracts from COS7 cells treated with PMA (125ng/ml, 30mins) with phosphopeptide

Anti-PKC zeta (phospho T560) antibody - ab192206 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC zeta (phospho T560) antibody (ab192206) at 1/500 dilutionLane 1 : PMA treated COS7 whole cell lysate with antigen-specific peptideLane 2 : PMA treated COS7 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-PKC zeta (phospho T560) antibody [EP2037AY] - ab62372 from Abcam

Prices $99.00, $390.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2037AY
All lanes : Anti-PKC zeta (phospho T560) antibody [EP2037AY] (ab62372)Lane 1 : Hela Cell Lysate (untreated with Calyculin A)Lane 2 : Hela Cell Lysate (Treated with Calyculin A)

Anti-PKC zeta antibody - ab59364 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
PKC zeta was immunoprecipitated using 0.5mg Mouse Brain tissue, 5µg of Rabbit polyclonal to PKC zeta and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59364.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Anti-Rabbit IgG light chain HRP (ab99697).Band: 68kDa; PKC zeta, non specific bands - 52kDa: We are unsure as to the identity of this extra band.

Anti-PKC zeta antibody - ab51157 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC zeta antibody (ab51157) at 1/500 dilutionLane 1 : extracts from 3T3 cells treated with 125ng/ml PMA for 30min, without immunizing peptideLane 2 : extracts from 3T3 cells treated with 125ng/ml PMA for 30min, with immunizing peptide

Anti-PKC zeta antibody - ab57432 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab57432 PKC zeta antibody at 1ug/ml.

Anti-PKC zeta antibody - ab71760 from Abcam

Prices $378.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PKC zeta antibody (ab71760) at 1/100 dilution + human placenta lysate at 12.5 µgSecondaryHRP-anti-rabbit

Anti-PKC zeta antibody - ab4139 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

Anti-PKC zeta antibody - ab89490 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKC zeta antibody (ab89490) at 1/1000 dilutionLane 1 : 3T6 cell lysate at 3 µgLane 2 : 3T6 cell lysate at 10 µgLane 3 : 3T6 cell lysate at 30 µg

Anti-PKC zeta antibody - ab117712 from Abcam

Prices $376.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Prostate, Human: Formalin-Fixed, Paraffin-Embedded tissue stained with ab117712 at 10µg/ml.

Anti-PKC zeta antibody [EP1490(2)] - ab108970 from Abcam

Prices $99.00, $379.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP1490(2)
ab108970 staining PKC zeta in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.See Abreview

Anti-PKC zeta antibody [S19-V] (FITC) - ab140017 from Abcam

Prices $376.00
Sizes 50 tests
Host Rabbit
Clonality Monoclonal
Clone S19-V
Flow cytometric analysis of peripheral blood lymphocytes labeling PKC zeta with ab140017 at a 1/50 dilution, compared with an isotype control.

Anti-PRKCZ / PKC-Zeta Antibody (phospho-Thr410) - LS-C336066 from LifeSpan Bioscience

Prices $285.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Phospho-PRKCZ-T410 antibody.

Anti-PRKCZ / PKC-Zeta Antibody (Cytoplasmic Domain) IHC-plus™ - LS-A8391 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-PRKCZ / PKC-Zeta antibody LS-A8391 IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Anti-PRKCZ / PKC-Zeta Antibody (aa577-592) - LS-C26196 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCZ / PKC-Zeta Antibody (Internal) - LS-C26198 from LifeSpan Bioscience

Prices $475.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-PRKCZ / PKC-Zeta Antibody - LS-C26200 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PRKCZ / PKC-Zeta Antibody (phospho-Thr410/Thr403) - LS-C26210 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
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