All lanes : Anti-PKLR antibody [1H9] (ab123908) at 1/2000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY controlLane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY PKLR cDNALysates/proteins at 5 µg per lane.
ab123908, at 1/100 dilution, staining PKLR in COS7 cells transiently transfected with pCMV6-ENTRY PKLR by Immunofluorescence.
HEK293T cells transfected with either a PKLR overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained using ab123908 at 1/100 dilution, and then analyzed by flow cytometry.