Anti-PKM2 antibody

Name	Anti-PKM2 antibody
Supplier	Abcam
Catalog	ab38237
Prices	$391.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Applications	ICC/IF ICC/IF IHC-P WB
Species Reactivities	Mouse, Human, Rat, Monkey
Antigen	Synthetic peptide corresponding to Human PKM2 aa 476-505 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH)
Description	Rabbit Polyclonal
Gene	PKM
Conjugate	Unconjugated
Supplier Page	Shop

Product images

Anti-PKM2 antibody (ab38237) at 1/100 dilution + HeLa cell lysate

Anti-PKM2 antibody (ab38237) + Ramos cell lysate at 35 µg

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling PKM2 with ab38237. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PKM2 with ab38237 at 1/50. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by ACE staining.

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling PKM2 (green) with ab38237. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Cells were incubated with the primary antibody (1/200, 2 h at room temperature). An Alexa Fluor® 488-conjugated donkey anti-rabbit antibody was used as the secondary antibody (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

ICC/IF image of ab38237 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

All lanes : Anti-PKM2 antibody (ab38237) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : PC-12 cell lysateLane 3 : Ramos cell lysateLane 4 : NIH/3T3 cell lysateLane 5 : Mouse brain tissue lysateLysates/proteins at 20 µg per lane.SecondaryHRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

Product References

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