PKM

Gene Symbol PKM
Entrez Gene 5315
Alt Symbol CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1
Species Human
Gene Type protein-coding
Description pyruvate kinase, muscle
Other Description OIP-3|OPA-interacting protein 3|PK, muscle type|cytosolic thyroid hormone-binding protein|epididymis secretory protein Li 30|p58|pyruvate kinase 2/3|pyruvate kinase PKM|pyruvate kinase isozymes M1/M2|pyruvate kinase muscle isozyme|thyroid hormone-binding protein 1|thyroid hormone-binding protein, cytosolic|tumor M2-PK
Swissprots Q53GK4 B2R5N8 Q9UCV6 B4DFX8 Q9UPF2 P14786 A6NFK3 B4DUU6 Q9BWB5 Q96E76 P14618 B3KRY0
Accessions AAD47248 AAD47249 AAF01766 AAF65764 AAQ15274 CAA39849 EAW77884 EAW77885 EAW77886 EAW77887 EAW77888 EAW77889 EAW77890 P14618 AF025439 AAC39559 AK092369 BAG52542 AK222927 BAD96647 AK294315 BAG57589 AK297951 BAG60266 AK299413 BAG61395 AK300800 BAG62458 AK307855 AK308264 AK309781 AK312253 BAG35185 BC000481 AAH00481 BC007640 AAH07640 BC007952 AAH07952 BC012811 AAH12811 BC019265 AAH19265 BC023328 BC023592 BC035198 AAH35198 BC035487 BC094767 AAH94767 BC096823 BG490968 BX648597 DA382029 DC376054 DQ892739 ABM83665 EU176742 ABW03543 EU794631 ACJ13685 EU832358 ACE87615 EU832443 ACE86928 M23725 AAA36449 M26252 AAA36672 Z36786 Z36831 XM_005254443 XP_005254500 XM_005254445 XP_005254502 XM_006720570 XP_006720633 XM_011521670 XP_011519972 XM_011521671 XP_011519973 XM_011521672 XP_011519974 XM_011521673 XP_011519975 NM_001206796 NP_001193725 NM_001206797 NP_001193726 NM_001206798 NP_001193727 NM_001206799 NP_001193728 NM_002654 NP_002645 NM_182470 NP_872270 NM_182471 NP_872271
Function Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. {ECO:0000269|PubMed:17308100, ECO:0000269|PubMed:18191611, ECO:0000269|PubMed:21620138}.
Subcellular Location Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
Tissue Specificity Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. {ECO:0000269|PubMed:18191611}.
Top Pathways Viral carcinogenesis, Glycolysis / Gluconeogenesis, Type II diabetes mellitus, Central carbon metabolism in cancer, Glucagon signaling pathway

PKM1 (D30G6) XP ® Rabbit mAb - 7067 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D30G6
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human PKM1 (hPKM1-Myc/DDK; +) or Myc/DDK-tagged full-length human PKM2 (hPKM2-Myc/DDK; +), using PKM1 (D30G6) XP ® Rabbit mAb (upper), DYKDDDDK (9A3) Mouse mAb #8146 (middle), or GAPDH (D16H11) XP ® Rabbit mAb #5174 (lower).

PKM1/2 (C103A3) Rabbit mAb - 3190 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C103A3
Western blot analysis of extracts from various cell types using PKM1/2 (C103A3) Rabbit mAb.

PKM1/2 Antibody - 3186 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extract from mouse muscle using PKM1/2 Antibody.

PKM1/2 (C5E6) Rabbit mAb - 3106 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C5E6
Western blot analysis of extracts from various cell types using PKM1/2 (C5E6) Rabbit mAb.

Phospho-PKM2 (Tyr105) Antibody - 3827 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
GST-PKM2 wild-type or the Tyr105Phe mutant was incubated in an in vitro kinase assay in the presence or absence of active FGFR1. Western blot analysis was performed using Phospho-PKM2 (Tyr105) Antibody and a phospho-Tyr antibody. The data demonstrate the specificity of the Phospho-PKM2 (Tyr105) Antibody and that the Tyr105Phe mutation abolishes PKM2 phosphorylation at Tyr105 by FGFR1 in vitro. (Adapted from Hitosugi, T. et al., 2009).

PKM2 (D78A4) XP ® Rabbit mAb - 4053 from Cell Signaling Technology

Prices $109.00, $277.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D78A4
Western blot analysis of extracts from various cell lines and mouse skeletal muscle using PKM2 (D78A4) XP ® Rabbit mAb (upper) or GAPDH (14C10) Rabbit mAb #2118.

PKM2 Antibody - 3198 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various tissue and cell lysates using PKM2 Antibody.

PKM2 (C-11) - sc-365684 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PKM2 (C-11): sc-365684. Western blot analysis of PKM2 expression in Jurkat (A) and HeLa (B) whole cell lysates.

PKM2 (E-12) - sc-65178 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PKM2 (H-59) - sc-292640 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKM2 (H-59): sc-292640. Western blot analysis of PKM2 expression in U-251 MG (A) and A549 (B) whole cell lysates.

PKM2 (D-15) - sc-65177 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

PKM2 (H-60) - sc-135048 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
PKM2 (H-60): sc-135048. Western blot analysis of PKM2 expression in U-251 MG whole cell lysate.

PKM2 (C-12) - sc-65176 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
PKM2 (C-12): sc-65176. Immunoperoxidase staining of formalin fixed, paraffin-embedded urinary bladder tissue showing cytoplasmic and nuclear staining of urothelial cells.

PKM2 (YY-3) - sc-100538 from Santa Cruz Biotechnology

Prices $295.00
Sizes 200 µl ascites
Host Mouse
Clonality Monoclonal
PKM2 (YY-3): sc-100538. Western blot analysis of PKM2 expression in K-562 whole cell lysate.

Anti-PKM2 (phospho Y105) antibody - ab156856 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKM2 (phospho Y105) antibody (ab156856) at 1 µg/mlLane 1 : F9 (Mouse embryonic carcinoma cell line) Whole Cell LysateLane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/mlLane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Control peptide at 1 µg/mlLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PKM2 antibody - ab38237 from Abcam

Prices $391.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PKM2 antibody (ab38237) at 1/100 dilution + HeLa cell lysate

Anti-PKM2 antibody - ab116271 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab116271 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab116271, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.

Anti-PKM2 antibody - ab137791 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKM2 antibody (ab137791) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : HeLa S3 whole cell lysate Lysates/proteins at 30 µg per lane.

Anti-PKM2 antibody - ab137852 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKM2 antibody (ab137852) at 1/1000 dilutionLane 1 : 293T whole cell lysateLane 2 : A431 whole cell lysateLane 3 : H1299 whole cell lysateLane 4 : HeLa S3 whole cell lysateLane 5 : Hep G2 whole cell lysateLane 6 : MOLT4 whole cell lysateLane 7 : Raji whole cell lysateLysates/proteins at 30 µg per lane.

Anti-PKM2 antibody - ab137857 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKM2 antibody (ab137857) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-PKM2 antibody - ab131021 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human osteosarcoma tissue labelling PKM2 with ab131021 at 1/1000 (1µg/ml). Detection: DAB.

Anti-PKM2 antibody - ab55602 from Abcam

Prices $387.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal

Anti-PKM2 antibody - ab85555 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PKM2 antibody (ab85555) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lane 6 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate Lane 7 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-PKM2 antibody - ab85542 from Abcam

Prices $376.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ab85542, at 1/100 dilution, staining PKM2 in formalin-fixed paraffin-embedded human tonsil tissue by Immunohistochemistry.

Anti-PKM2 antibody - ab135638 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-PKM2 antibody (ab135638) at 1/50 dilution + CEM cell line lysate at 35 µg
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