All lanes : Anti-PKM2 antibody (ab137791) at 1/1000 dilutionLane 1 : A431 whole cell lysateLane 2 : H1299 whole cell lysateLane 3 : HeLa S3 whole cell lysate Lysates/proteins at 30 µg per lane.
Anti-PKM2 antibody (ab137791) at 1/10000 dilution + Mouse brain whole cell lysate at 50 µg
Immunofluorescence analysis of methanol-fixed HeLa cells labelling PKM2 with ab137791 at 1/500 dilution. The lower image is co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin embedded Human Cal27 xenograft tissue labelling PKM2 with ab137791 at 1/500 dilution.